JCRB1642 KMS-11/BTZ

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1642 Cell Name   KMS-11/BTZ
Profile   bortezomib-resistant multiple myeloma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    F Age   67-year-old
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   pleural effusion formation Metastasis   
Tissue Metastasized    Genetics   t(4;14)(p16.3;q32.3)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   small round-cell morphology, part is multinucleate cell.
Classify   tumor Established by   Nakashima, T.
Registered by   Hasegawa, M. Regulation for Distribution   
Comment    Year   2015
Medium   RPMI1640 medium with 10% featal bovine serum. Methods for Passages   Subculture twice a week by dilution.
Cell Number on Passage   50000 cells / mL Race   
CO2 Conc.   5% Tissue Sampling   pleural effusion
Tissue Type   myeloma pleural effusion infiltration (IgGk)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4V71
Reference
Pubmed id:20555361Bortezomib-resistant myeloma cell lines: a role for mutated PSMB5 in preventing the accumulation of unfolded proteins and fatal ER stress.
Ri M,Iida S,Nakashima T,Miyazaki H,Mori F,Ito A,Inagaki A,Kusumoto S,Ishida T,Komatsu H,Shiotsu Y,Ueda R
Leukemia. 2010 Aug;24(8):1506-12

Images
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1642 Cell Name   KMS-11/BTZ
LOT No.   09292015 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.5-6.0x10^5 cells/ml Methods for Passages   dilution,(1/2-1/6)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.10x10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:12
D7S820:11,12
D16S539:9,13
VWA:17,18
TH01:6,9
AM:X
TPOX:12
CSF1PO:13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1642 Cell Name   KMS-11/BTZ
LOT No.   07092020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.5 - 3.1 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 35 hrs Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   88.7 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   85.4 Additional information   
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