JCRB1635 ITSM
Cell information
Important Notice(s)Announcement of unavailability of this cell line to overseas researcher outside of Japan
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1635 | Cell Name | ITSM |
|---|---|---|---|
| Profile | Human B cell line established from patient diagnosed as pseudomyxoma peritonei. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 70-year-old |
| Identity | available | Tissue for Primary Cancer | cecum |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | floating agglutination | Character | CD20(+), CD30(+), CD79a(+), Bcl2(+), EBER(+), EBNA-2(+), Ig?-mRNA(+), LMP-1(+), cCD3, CD44(+), CD45(+), CD68(-), CD133(-), MUC2(-), MUC5AC(-), cCD3(-), Ig?mRNA(-) |
| Classify | tumor | Established by | Shimada, Y. |
| Registered by | Shimada, Y. | Regulation for Distribution | |
| Comment | Year | 2015 | |
| Medium | RPMI1640/Ham's F12 medium with 5% FBS | Methods for Passages | dilution (Split ratio 1/5 - 1/10) |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | ascites |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | + | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1635 | Cell Name | ITSM |
|---|---|---|---|
| LOT No. | 07312015 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) and Ham's F12 medium(SIGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.56-6.7x10^5 cells/ml | Methods for Passages | dilution,(1/5-1/10) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.45x10^6 |
| Viability at cell freezing (%) | 96.60 | Antibiotics Used | free |
| Passage Number | p3* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:9,11 D7S820:11,12 D16S539:10,11 VWA:14,17 TH01:9 AM:X TPOX:8,11 CSF1PO:9,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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