JCRB0709.2 L5178Y TK+/-3.7.2c(IVGT)

Cell information

Important Notice(s)

Growth property of L5178Y TK+/-

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0709.2 Cell Name   L5178Y TK+/-3.7.2c(IVGT)
Profile   Mouse TK+/- heterozygotic subline of the L5178Y. Other Name   
Animal   mouse Strain   DBA/2
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   hemo-lymphocytic
Case history   lymphoma induced by methylcholanthrene treatment Metastasis   
Tissue Metastasized    Genetics   leukemia
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   TK+/- heterozygotic subline of the L5178Y
Classify   transformed Established by   
Registered by    Regulation for Distribution   No regulation was applied to this strain to establisher.
Comment   Incomplete description. Year   2003
Medium   Fischer's medium with 10% horse serum. Methods for Passages   Suspend cells by pipetting.
Cell Number on Passage   2-3 x10^4 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   lymph node
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W65
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0709.2 Cell Name   L5178Y TK+/-3.7.2c(IVGT)
LOT No.   12H013 Lot Specification   distribution
Medium   Fischer's medium with 10% horse serum. Temperature   
Cell Density at Seeding   2-3 x10^4 cells/ml Methods for Passages   Suspend cells by pipetting.
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA    Sterility: BACTERIA   
Sterility: FUNGI    Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   The L5178Y tk+/- 3.7.2c (IVGT) mouse lymphoma cell line was derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene treatment. The cell line is tumourigenic in DBA/2 mice. The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction.
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