JCRB1612 HuS-L12/10

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1612 Cell Name   HuS-L12/10
Profile   Human immortalized cell line derived from MRC-5 cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   fetus
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Imai, S.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2013
Medium   Basal medium Eagle with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   Lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG29
Reference
Pubmed id:10799292PCCX1, a novel DNA-binding protein with PHD finger and CXXC domain, is regulated by proteolysis.
Fujino T,Hasegawa M,Shibata S,Kishimoto T,Imai S,Takano T
Biochem Biophys Res Commun. 2000 May 10;271(2):305-10
Pubmed id:9398664Dissociation of Oct-1 from the nuclear peripheral structure induces the cellular aging-associated collagenase gene expression.
Imai S,Nishibayashi S,Takao K,Tomifuji M,Fujino T,Hasegawa M,Takano T
Mol Biol Cell. 1997 Dec;8(12):2407-19
Pubmed id:7935433Immortalization-susceptible elements and their binding factors mediate rejuvenation of regulation of the type I collagenase gene in simian virus 40 large T antigen-transformed immortal human fibroblasts.
Imai S,Fujino T,Nishibayashi S,Manabe T,Takano T
Mol Cell Biol. 1994 Nov;14(11):7182-94
Pubmed id:8397328Escape from in vitro aging in SV40 large T antigen-transformed human diploid cells: a key event responsible for immortalization occurs during crisis.
Imai S,Saito F,Ikeuchi T,Segawa K,Takano T
Mech Ageing Dev. 1993 Jun;69(1-2):149-58
Pubmed id:1280416Loss of collagenase gene expression in immortalized clones of SV40 T antigen-transformed human diploid fibroblasts.
Imai S,Takano T
Biochem Biophys Res Commun. 1992 Nov 30;189(1):148-53

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1612 Cell Name   HuS-L12/10
LOT No.   11042014 Lot Specification   distribution
Medium   Basal Medium Eagle(SIGMA) with 2mM L-Glutamine and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.05-4.55x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.31x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:11,14
D7S820:10,11
D16S539:9,11
VWA:15
TH01:8
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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