JCRB1601 EMTOKA

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1601 Cell Name   EMTOKA
Profile   Human cell line derived from uterine carcinosarcoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   64-year-old
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   The cells transplantable to nude mice.
Classify   tumor Established by   Gorai, I.
Registered by   Gorai, I. Regulation for Distribution   
Comment    Year   2013
Medium   RPMI1640 medium with 10% fetal bovine serum Methods for Passages   Cells are harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Cell Number on Passage   1-3 x 10^4 cells/mL Race   
CO2 Conc.   5% Tissue Sampling   Uterine body
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_E115
Reference
Pubmed id:9311600Uterine carcinosarcoma is derived from a single stem cell: an in vitro study.
Gorai I,Yanagibashi T,Taki A,Udagawa K,Miyagi E,Nakazawa T,Hirahara F,Nagashima Y,Minaguchi H
Int J Cancer. 1997 Sep 4;72(5):821-7
Pubmed id:8431860Establishment and characterization of carcinosarcoma cell line of the human uterus.
Gorai I,Doi C,Minaguchi H
Cancer. 1993 Feb 1;71(3):775-86

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1601 Cell Name   EMTOKA
LOT No.   08052014 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.57-2.65x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   nT Cell Number in Vial (cells/1ml)   1.23x10^6
Viability at cell freezing (%)   95.1 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,11
D13S317:11,13
D7S820:10
D16S539:9,12
VWA:17,18
TH01:9
AM:X
TPOX:8,11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1601 Cell Name   EMTOKA
LOT No.   11242020 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   3.9 - 5.2 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   97 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
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