JCRB1590 TYGBK-8
Cell information
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Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1590 | Cell Name | TYGBK-8 |
|---|---|---|---|
| Profile | Human gall bladder cancer cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 38-year-old |
| Identity | available | Tissue for Primary Cancer | gallbladder |
| Case history | gallbladder cancer (pancreaticobiliary maljunction) | Metastasis | |
| Tissue Metastasized | Genetics | p53 mutation exon 7 codon 237 (ATG to ATA?, no K-Ras mutation in codon 12 or 13 | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | moderately differentiated tubular carcinoma, T2N1M1, stage4b |
| Classify | tumor | Established by | Shimada, Y. |
| Registered by | Shimada, Y. | Regulation for Distribution | |
| Comment | Year | 2014 | |
| Medium | Ham's F12 medium + RPMI1640 (1 to 1 mix) with 5% fetal calf serum (cultued by collagen coat dish). | Methods for Passages | Cells harvested after treatment with trypsin and EDTA. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | gall bladder |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Movies |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1590 | Cell Name | TYGBK-8 |
|---|---|---|---|
| LOT No. | 06272014 | Lot Specification | distribution |
| Medium | RPMI1640 and Ham's F12 medium (1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911)and using BioCoat Collagen 1 100mm Dish(Becton Dickinson #354450 or #356450) | Temperature | 37 C |
| Cell Density at Seeding | 1.33-1.58x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.23x10^6 |
| Viability at cell freezing (%) | 97.2 | Antibiotics Used | |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:11 D7S820:11,12 D16S539:9 VWA:17,19 TH01:8 AM:X TPOX:8,11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |