JCRB1588 ACI-RCC-ffLuc

Cell information

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1588 Cell Name   ACI-RCC-ffLuc
Profile   Luc expressing rat renal cell carcinoma cell line. Other Name   
Animal   rat Strain   ACI
Genus   Rattus Species   norvegicus
Sex    Age   
Identity   not done Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   transformed by pMEI-5Neo (pGL3 for ffLuc)
Life Span   infinite Crisis PDL   
Morphology   Spindle-shape Character   Semi-floating cells. G418-resistant.
Classify   transformed Established by   Kobayashi, M.
Registered by   Kobayashi, M. Regulation for Distribution   
Comment    Year   2013
Medium   DMEM with 10% FBS Methods for Passages   Pipetting
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG46
Reference
Pubmed id:20303523Establishment and characterization of transplantable, luminescence labeled rat renal cell carcinoma cell lines.
Kobayashi M,Murakami T,Uchibori R,Chun NA,Kobayashi E,Morita T,Ozawa K
J Urol. 2010 May;183(5):2029-35

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1588 Cell Name   ACI-RCC-ffLuc
LOT No.   06042014 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911),culturing in BD Falcon 75 sq.cm Canted Neck Culture Flask(Cat.No.353136) Temperature   37 C
Cell Density at Seeding   0.51-1.47x10^5 cells/ml Methods for Passages   Adherent and non-adherent cells exist.Collect both types of cells. The adherent cells were harvested by pipetting.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.88x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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