JCRB1554 MMNK-1

Cell information

Important Notice(s)

This cell line is available for only academic users.

本細胞は学術機関へのみの分譲となります

Articles regarding TMNK-1 / TWNT-1 / MMNK-1

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1554 Cell Name   MMNK-1
Profile   Highly differentiated immortalized human cholangiocyte cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Expressing catalytic subunit of human telomerase reverse transcriptase (hTERT) and enhanced green fluorescent protein (EGFP).
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Kobayashi, N.
Registered by   Mochitate, K. Regulation for Distribution   [Maruyama et al. Transplantation 77: 446-451, 2004] should be cited at the paper submission.
Comment   The distribution is limieted to academic Japan domestic users and academic oversea users. The following reference should be cited at the publication of paper. Maruyama et al. Transplantation 77: 446-451, 2004. Year   2013
Medium   DMEM with 5% fetal bovine serum Methods for Passages    Cells were harvested after treatment with Accutase and split ratio(1/3-1/4)
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   cholangiocytes (OUMS-21)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_M266
Reference
Pubmed id:14966424Establishment of a highly differentiated immortalized human cholangiocyte cell line with SV40T and hTERT.
Maruyama M,Kobayashi N,Westerman KA,Sakaguchi M,Allain JE,Totsugawa T,Okitsu T,Fukazawa T,Weber A,Stolz DB,Leboulch P,Tanaka N
Transplantation. 2004 Feb 15;77(3):446-51

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1554 Cell Name   MMNK-1
LOT No.   09142013 Lot Specification   distribution
Medium   DMEM with 5% fetal bovine serum(SIGMA027K03911,non HI) Temperature   37 C
Cell Density at Seeding   0.4-1.5x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with Accutase and split ratio(1/3-1/4)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.15x10^6
Viability at cell freezing (%)   97.0 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,15
D13S317:9,12
D7S820:8,9
D16S539:11,13
VWA:14,17
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1554 Cell Name   MMNK-1
LOT No.   02022017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   5.3 - 6.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with Accutase.
Doubling Time   approx. 32 hrs Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   84.9 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   84.9 Additional information   
Cell No.   JCRB1554 Cell Name   MMNK-1
LOT No.   05162019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   0.3 - 1.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with Accutase.
Doubling Time   approx. 26 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   96.9 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.9 Additional information   
Cell No.   JCRB1554 Cell Name   MMNK-1
LOT No.   07042024 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 5% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.6 - 3.1 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with accutase.
Doubling Time   20 - 30 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   93.7 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,15
D13S317:9,12
D7S820:8,9
D16S539:11,13
VWA:14,17
TH01:7,9
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   83.8 Additional information   
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