JCRB1550 HAdpc-25-Bmi-1-TERT

Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1550 Cell Name   HAdpc-25-Bmi-1-TERT
Profile   Immortalized human adipose tissue-derived mesenchymal stem cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   adipose tissue
Case history    Metastasis   No
Tissue Metastasized    Genetics   Human adipose tissue-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the Bmi-1 and hTERT.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Derived from subcutaneous adipose tissue around mammary gland. hTERT and bmi-1 genes were transfected.
Classify   transformed Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2011
Medium   Poweredby 10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   adipocyte

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W37
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1550 Cell Name   HAdpc-25-Bmi-1-TERT
LOT No.   10232013 Lot Specification   distribution
Medium   POWEREDBY10(within serum )catalog No.6012(Glycotechnica) Temperature   37 C
Cell Density at Seeding   0.5-1.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.52x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   
Passage Number   p37 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11
D7S820:11,12
D16S539:9,13
VWA:17,19
TH01:7
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1550 Cell Name   HAdpc-25-Bmi-1-TERT
LOT No.   09122019 Lot Specification   distribution
Medium   POWEREDBY10（GlycoTechnica Ltd., catalog No.6012). Within serum. Temperature   37 C
Cell Density at Seeding   1.0 - 2.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.7 x 10^5
Viability at cell freezing (%)   approx. 10 Antibiotics Used   free
Passage Number   P40 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.8 Additional information   
Cell No.   JCRB1550 Cell Name   HAdpc-25-Bmi-1-TERT
LOT No.   09182024 Lot Specification   distribution
Medium   POWEREDBY10(within serum) Temperature   37 C
Cell Density at Seeding   0.6-2.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   100.00 Antibiotics Used   free
Passage Number   p47 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11
D7S820:11,12
D16S539:9,13
VWA:17,19
TH01:7
AM:X
TPOX:8,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   95.5 Additional information   
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