JCRB1548 H-1A(H123)

Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1548 Cell Name   H-1A(H123)
Profile   Mouse bone marrow-derived osteoblast-like cell line. Other Name   
Animal   mouse Strain   C57/BL
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   polygonal-fibroblast Character   
Classify   normal Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2011
Medium   Poweredby 10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   osteoblast-like cell

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W36
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1548 Cell Name   H-1A(H123)
LOT No.   09102013 Lot Specification   distribution
Medium   POWEREDBY10 Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.(Split ratio=1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.07x10^6
Viability at cell freezing (%)   94.9 Antibiotics Used   
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Mouse confirmed by PCR method.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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