JCRB1512 TYUC-1

Cell information

Important Notice(s)

Announcement of unavailability of this cell line to overseas researcher outside of Japan

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1512 Cell Name   TYUC-1
Profile   Human esophageal small cell carcinoma cell line established from mouse xenograft. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   56-year-old
Identity   available Tissue for Primary Cancer   esophagus
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   floating Character   undifferentiated carcinoma, T4M1N0 stage 3
Classify   tumor Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2012
Medium   Ham's F12 medium + DMEM (1 to 1 mix) with 5% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   mouse xenograft
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W84
Reference
Pubmed id:25667451MicroRNA profiles to predict postoperative prognosis in patients with small cell carcinoma of the esophagus.
Okumura T,Shimada Y,Omura T,Hirano K,Nagata T,Tsukada K
Anticancer Res. 2015 Feb;35(2):719-27

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1512 Cell Name   TYUC-1
LOT No.   02122013 Lot Specification   distribution
Medium   DMEM and Ham's F12 medium(SiGMA)(1/1) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1-5x10^5 cells/ml Methods for Passages   Dilution,split 1/2-1/4.(Centrifuge at 90-100 xg for 3 min to remove dead cells)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.56x10^6
Viability at cell freezing (%)   66.5 Antibiotics Used   
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8
D7S820:10
D16S539:11
VWA:16
TH01:9
AM:X
TPOX:8
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1512 Cell Name   TYUC-1
LOT No.   05202019 Lot Specification   distribution
Medium   1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.6 - 1.8 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture), split ratio 1/2 - 1/4.
Doubling Time    Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM/F12 CO2 Conc.   5%
Viability immediately after thawing (%)   83.3 Additional information   The cells will grow with forming cell aggregates, and many dead cells will appear during culture.
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