JCRB1459 HUEhT-2

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1459 Cell Name   HUEhT-2
Profile   Immortalized human umbilical vein endothelial cell line established (HUVEC) by electroporation of pIRES-hTERT-hygr. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   HUEhT-2 was established by electroporation of pIRES-hTERT-hygr.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Cell culture in collagen I coated dish.
Classify   transformed Established by   Anno, K.
Registered by   Anno, K. Regulation for Distribution   
Comment    Year   2011
Medium   MCDB131 medium supplemented with 10% fetal bovine serum, 0.03g/L endothelial cell growth supplement (ECGS), 5ug/mL heparin, and 10mM L-glutamine. Methods for Passages   trypsinization with 0.025% trypsin- 0.265mM EDTA
Cell Number on Passage   split ratio = 1:4 Race   
CO2 Conc.   5% Tissue Sampling   human umbilical vein
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W52
Reference
Pubmed id:17207773Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells.
Anno K,Hayashi A,Takahashi T,Mitsui Y,Ide T,Tahara H
Biochem Biophys Res Commun. 2007 Feb 23;353(4):926-32

Images
PDF
   PDF File1
Movies



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1459 Cell Name   HUEhT-2
LOT No.   03242015 Lot Specification   distribution
Medium   MCDB131(GIBCO)medium with 10mM Glutamine,0.03g/L ECGS,5mg/L Heparin-Na and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.0-3.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.01% EDTA4Na.(split ratio=1/4) (collagen type I coated dish)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.67x10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   
Passage Number   p7* PDL   74
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1459 Cell Name   HUEhT-2
LOT No.   02272012 Lot Specification   distribution
Medium   MCDB131(GIBCO)medium with 10mM Glutamine,0.03g/L ECGS,5mg/L Heparin-Na and 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.0-3.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.01% EDTA4Na.(split ratio=1/4) (collagen type I coated dish)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.39x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   p4* PDL   67.6
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1459 Cell Name   HUEhT-2
LOT No.   02062018 Lot Specification   distribution
Medium   MCDB131(GIBCO)medium with 10mM Glutamine(Sigma),0.03g/L ECGS,5mg/L Heparin-Na and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.01% EDTA・4Na(split ratio=1/4) (collagen type I coated dish)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.56x10^6
Viability at cell freezing (%)   96.4 Antibiotics Used   free
Passage Number   p7* PDL   74.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB1459 Cell Name   HUEhT-2
LOT No.   02032020 Lot Specification   distribution
Medium   MCDB131(GIBCO)medium with 10mM Glutamine(Sigma),0.03g/L ECGS,5mg/L Heparin-Na and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.01% EDTA4Na.(split ratio=1/4) (collagen type I coated dish)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.59x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p7* PDL   74.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB1459 Cell Name   HUEhT-2
LOT No.   06152021 Lot Specification   distribution
Medium   MCDB131(GIBCO)medium with 10mM Glutamine(Sigma),0.03g/L ECGS,5mg/L Heparin-Na and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   6.76-8.65x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.01% EDTA4Na.(split ratio=1/4) (collagen type I coated dish)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.63x10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   p7* PDL   75.5
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:8
D7S820:12
D16S539:10
VWA:17,19
TH01:6
AM:X
TPOX:8,9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   85.7 Additional information   
Images
Cell No.   JCRB1459 Cell Name   HUEhT-2
LOT No.   04092025 Lot Specification   distribution
Medium   MCDB131(GIBCO)medium with 10mM Glutamine, 0.03g/L ECGS, 5mg/L heparin-Na and 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 7 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.01% EDTA.(split ratio=1/4). Use collagen type I coated dish.
Doubling Time   43 - 69 hrs. Cell Number in Vial (cells/1ml)   9.5 x 10^5
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   79
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   95.1 Additional information   
CLOSE