JCRB1453 CLAC

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1453 Cell Name   CLAC
Profile   Canine lung adenocarcinoma cell line including cancer stem cells (0.75%) in sidepopulation (SP). Other Name   
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   M Age   10-year-old
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   This cell line including cancer stem cells (0.75%).
Classify   tumor Established by   Sahara, H.
Registered by   Sahara, H. Regulation for Distribution   
Comment    Year   2011
Medium   DMEM medium with 10% FBS, 1% NEAA, 0.29mg/mL L-glutamine. Methods for Passages   Trypsin-EDTA
Cell Number on Passage   split 1/3 Race   
CO2 Conc.   5% Tissue Sampling   lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J360
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1453 Cell Name   CLAC
LOT No.   01312012 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) ,1%NEAA and 0.29 mg/ml L-glutamine. Temperature   37 C
Cell Density at Seeding   0.36-1.9x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.Split ratio=1/3.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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