JCRB9113 HT-1080

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9113 Cell Name   HT-1080
Profile   Human sarcoma cell line with activated N-ras oncogne. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   35
Identity   available Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   The cells contain activated N-ras oncogene.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Rasheed,S. st al.
Registered by    Regulation for Distribution   
Comment    Year   1987
Medium   Eagle's Minimal Essential medium with non -essential amino acids and 10% fetal calf serum. Methods for Passages   Treat cells with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   dilute 1/4 - 1/8 Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   fibrosarcoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0317
Reference
Pubmed id:2125632Synergistic antitumor effect of glycosylated recombinant human lymphotoxin with human interferon-gamma on lymphotoxin-sensitive human tumor.
Kawatsu M,Funahashi I,Kajikawa T,Takeo K,Asahi T,Yamashita T,Kawaharada H,Watanabe K
J Interferon Res. 1990 Oct;10(5):519-29
Pubmed id:841332Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:4132053Characterization of a newly derived human sarcoma cell line (HT-1080).
Rasheed S,Nelson-Rees WA,Toth EM,Arnstein P,Gardner MB
Cancer. 1974 Apr;33(4):1027-33


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Harada M,Morimoto K,Kondo T,Hiramatsu R,Okina Y,Muko R,Matsuda I,Kataoka T
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Pubmed id:24970122 Ursolic Acid Inhibits Na+/K+-ATPase Activity and Prevents TNF-α-Induced Gene Expression by Blocking Amino Acid Transport and Cellular Protein Synthesis.
Yokomichi T,Morimoto K,Oshima N,Yamada Y,Fu L,Taketani S,Ando M,Kataoka T
Biomolecules. 2011 Nov 7;1(1):32-47

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9113 Cell Name   
LOT No.   12032009 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids (0.1 mM) and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 2.7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA, passage twice a week.
Doubling Time   approx. 22 hrs Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9113 Cell Name   
LOT No.   10232003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2-5 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   D.T. = 21 hrs Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   89.0 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture meidum CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9113 Cell Name   HT-1080
LOT No.   040495 Lot Specification   distribution
Medium   Eagle's minimal essential medium with Non-essential AAs and 10% fetal bovine serum(JRHB 1B1062) Temperature   37 C
Cell Density at Seeding   1x10^5 cells/ml Methods for Passages   Harvest cells with 0.25% trypsin and 0.02% EDTA at 37 C for 1 min.
Doubling Time   24.0 hrs Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   P22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:12,14
D7S820:9,10
D16S539:9,12
VWA:14,19
TH01:6
AM:XY
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9113 Cell Name   HT-1080
LOT No.   052292 Lot Specification   distribution
Medium   E.MEM + NEAA (lot#.041592) + FBS 10% (Cell CultureLaboratories, lot#00939-01) Temperature   37 C
Cell Density at Seeding   8.3x10^5 Methods for Passages   0.25% trypsin in 0.02 % EDTA solution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.73x10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   P22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9113 Cell Name   HT-1080
LOT No.   092890 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/4 - 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9113 Cell Name   HT-1080
LOT No.   102188 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/4 - 1/8 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9113 Cell Name   
LOT No.   092596 Lot Specification   distribution
Medium   Eagle's MEM with non-esseitial amino acids and 10%fetal calf serum (FCS lot; BioWhittaker 3M0581) Temperature   37 C
Cell Density at Seeding   4-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9113 Cell Name   
LOT No.   060299 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non essential amino acids and 10% fetal calf serum. Temperature   37 C
Cell Density at Seeding   3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 32 hrs Cell Number in Vial (cells/1ml)   5.1 x 10^5
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9113 Cell Name   HT-1080
LOT No.   06072016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.6 - 3.9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 16 hrs Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   85.4 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   85.4 Additional information   
Cell No.   JCRB9113 Cell Name   HT-1080
LOT No.   03212021 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-080) with non essential amino acid (Wako) and 10% fetal bovine serum(SIGMA cat.No.172012 LotNo.12J396,non HI) Temperature   37 C
Cell Density at Seeding   1-1.2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. 1/9 split every 3days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.97x10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   p23 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:12,14
D7S820:9,10
D16S539:9,12
VWA:14,19
TH01:6
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   1.5x10^6 Additional information   
Images
Cell No.   JCRB9113 Cell Name   HT-1080
LOT No.   03132024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 4 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA,
Doubling Time   approx. 14 hrs. Cell Number in Vial (cells/1ml)   2.3 x 10^6
Viability at cell freezing (%)   93.5 Antibiotics Used   free
Passage Number   P23 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,13
D13S317:12,14
D7S820:9,10
D16S539:9,12
VWA:14,19
TH01:6
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM wtih NEAA CO2 Conc.   5%
Viability immediately after thawing (%)   91.8 Additional information   
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