JCRB1401 Kasumi-7

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1401 Cell Name   Kasumi-7
Profile   B cell leukemia Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   29
Identity   available Tissue for Primary Cancer   
Case history   acute lymphoblastic leukemia Metastasis   
Tissue Metastasized    Genetics   del(9p), [der(9)t(9;9)(p13;?),der(4)t(4;9)(q21;?)]
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   CD10+, CD19+, CD20+, HLA-DR+, CD33+
Classify   tumor Established by   Asou, H.
Registered by   Asou, H. Regulation for Distribution   
Comment    Year   2009
Medium   RPMI1640 medium with 20% featal bovine serum. Methods for Passages   dilution (split ratio 1:2)
Cell Number on Passage   0.5-2.0 x 10^6 cells Race   
CO2 Conc.   5% Tissue Sampling   blood
Tissue Type   tumor

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8486
Reference
Pubmed id:32180206Kasumi leukemia cell lines: characterization of tumor genomes with ethnic origin and scales of genomic alterations.
Kasai F,Asou H,Ozawa M,Kobayashi K,Kuramitsu H,Satoh M,Kohara A,Kaneko Y,Kawamura M
Hum Cell. 2020 Jul;33(3):868-876

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1401 Cell Name   Kasumi-7
LOT No.   04122011 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA Cat. # F0926, Lot 027K03911) Temperature   37 C
Cell Density at Seeding   2.97-8.45x10^5 cells/ml Methods for Passages   Dilution (split ratio=1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.42x10^6
Viability at cell freezing (%)   91.6 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,10
D7S820:9,10
D16S539:9
VWA:14,18
TH01:6
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1401 Cell Name   Kasumi-7
LOT No.   09062022 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   5-7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). Split ratio 1/2 is recommended.
Doubling Time   34 - 44 hrs. Cell Number in Vial (cells/1ml)   9.3 x 10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12,13
D13S317:8,10
D7S820:9,10
D16S539:9
VWA:14,18
TH01:6
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   90 Additional information   
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