JCRB9101 CCD-14Br

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9101 Cell Name   CCD-14Br
Profile   bronchus, diploid Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   lung
Case history    Metastasis   
Tissue Metastasized    Genetics   bronchus, diploid
Life Span    Crisis PDL   
Morphology    Character   bronchus, diploid
Classify    Established by   
Registered by    Regulation for Distribution   
Comment   Prepared from CCL203. Population doubling was 15 at passage 5. Year   1986
Medium   MEM w/ non essential amino acids + FCS 10% Methods for Passages   0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/2 - 1/3 Race   
CO2 Conc.   5 % Tissue Sampling   bronchiole
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2870
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9101 Cell Name   
LOT No.   10172003 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   0.6 - 1.0 x 10^4 cells/sq.cm Methods for Passages   0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time   D.T. = ca. 1.6 days Cell Number in Vial (cells/1ml)   6.1 x 10^5
Viability at cell freezing (%)   92.7 Antibiotics Used   free
Passage Number   P10 PDL   27
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9101 Cell Name   CCD-14Br
LOT No.   061689 Lot Specification   distribution
Medium   MEM w/ non essential amino acids + FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   79% Antibiotics Used   free
Passage Number   P10 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9101 Cell Name   CCD-14Br
LOT No.   04052019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2 - 1.5 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 39 hrs. Cell Number in Vial (cells/1ml)   5.0 x 10^5
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P10 PDL   28.4
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   88.1 Additional information   
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