JCRB1392 AS17

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1392 Cell Name   AS17
Profile   Hybridoma cell line producing antibody against human annexin IV. Other Name   
Animal   mouse x mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing mouse IgG 2b and kappa
Classify   hybridoma Established by   Kina T.
Registered by   Kina T. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI-1640 medium (10% FBS, 2mM Glutamine, 50uM 2-ME) Methods for Passages   dilution
Cell Number on Passage   Split 1/20 (1/10 - 1/20) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   Producing Mab human annexin IV.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8536
Reference
Pubmed id:9084877Characterization of human p33/41 (annexin IV), a Ca2+ dependent carbohydrate-binding protein with monoclonal anti-annexin IV antibodies, AS11 and AS17.
Satoh A,Takayama E,Kojima K,Ogawa H,Katsura Y,Kina T,Matsumoto I
Biol Pharm Bull. 1997 Mar;20(3):224-9

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1392 Cell Name   AS17
LOT No.   01242011 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 2mM Glutamine,50uM　2-ME and 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.506-3.84x10^5 cells/ml Methods for Passages   split 1/20(1/10-1/20)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.60x10^6
Viability at cell freezing (%)   84.1 Antibiotics Used   NT
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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