JCRB1385 CAS-9
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1385 | Cell Name | CAS-9 |
|---|---|---|---|
| Profile | Hybridoma cell line producing antibody against mouse CD49d (VLA-4 a chain). Binding inhibition VLA-4/Fibronectin. | Other Name | |
| Animal | mouse x rat | Strain | |
| Genus | Hybridoma | Species | |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Producing rat IgG 2b and kappa |
| Classify | hybridoma | Established by | Kina T. |
| Registered by | Kina T. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI-1640 medium (10% FBS, 2mM Glutamine, 50uM 2-ME) | Methods for Passages | dilution |
| Cell Number on Passage | Split 1/20 (1/10 - 1/20) | Race | |
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | Producing Mab mouse CD49d (VLA-4 a chain) |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:8660794 | Requirement of cell interactions through adhesion molecules in the early phase of T cell development. Wada K,Kina T,Kawamoto H,Kondo M,Katsura Y Cell Immunol. 1996 May 25;170(1):11-9 |
| Images |
|---|
      |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1385 | Cell Name | CAS-9 |
|---|---|---|---|
| LOT No. | 12092010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 2mM Glutamine,50uM 2-ME and 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.418-1.61x10^5 cells/ml | Methods for Passages | Split 1/20(1/10-1/20) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.54x10^6 |
| Viability at cell freezing (%) | 85.9 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
|---|
|
|