JCRB1384 STR-428
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1384 | Cell Name | STR-428 |
|---|---|---|---|
| Profile | Human HHV-8 negative malignant effusion lymphoma (DLBCL). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 53 |
| Identity | available | Tissue for Primary Cancer | ascites |
| Case history | Ascites lymphoma | Metastasis | |
| Tissue Metastasized | Genetics | IgH, c-Myc, BCL2 rearrangements, complicated karyotypes including t(14:18)(q32;q21), der(8)t(3;8)(q27;q24) | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Immunophenotypic characterization; CD3-,CD4-,CD5-,CD8-,CD10+,CD19+,CD20+,CD22+,CD30-,CD38+,HLA-DR+, Viral status; HIV(-), HHV-8(-), EBV(-). |
| Classify | tumor | Established by | Tomoyose,T. |
| Registered by | Masuda,M. | Regulation for Distribution | |
| Comment | Contact to Dr. Taira,T. to get information of this cell line. | Year | 2007 |
| Medium | RPMI1640 medium with 10% fetal bovine serum. | Methods for Passages | * |
| Cell Number on Passage | * | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | ascites |
| Tissue Type | large B cell lymphoma (DLBC) |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:17306873 | Lack of mitochondrial depolarization by oxidative stress is associated with resistance to buthionine sulfoximine in acute lymphoblastic leukemia cells. Goto H,Yanagimachi M,Kajiwara R,Kuroki F,Yokota S Leuk Res. 2007 Sep;31(9):1293-301 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1384 | Cell Name | STR-428 |
|---|---|---|---|
| LOT No. | 12092010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.44-2.4x10^5 cells/ml | Methods for Passages | subculture by dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.1x10^6 |
| Viability at cell freezing (%) | 78.4 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13 D13S317:11,12 D7S820:11 D16S539:9,12 VWA:14 TH01:6,9 AM:X,Y TPOX:8 CSF1PO:11 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1384 | Cell Name | STR-428 |
|---|---|---|---|
| LOT No. | 05182020 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 3.5 - 5.2 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). Cells will form aggregates. At passage, please disperse the cells by pipetting. |
| Doubling Time | approx. 15 hrs. | Cell Number in Vial (cells/1ml) | 3.2 x 10^6 |
| Viability at cell freezing (%) | 90.6 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | 87.3 | Additional information |