JCRB1370 TAS-5
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1370 | Cell Name | TAS-5 |
|---|---|---|---|
| Profile | Hybridoma cell line producing antibody against mouse CD49d (VLA-4 a chain). Binding inhibition VLA-4/VCAM-1 and VLA-4/Fibronectin | Other Name | |
| Animal | mouse x rat | Strain | |
| Genus | Hybridoma | Species | |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Producing rat IgG 2b and kappa |
| Classify | hybridoma | Established by | Kina T. |
| Registered by | Kina T. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI-1640 medium (10% FBS, 2mM Glutamine, 50uM 2-ME) | Methods for Passages | dilution |
| Cell Number on Passage | Split 1/20 (1/10 - 1/20) | Race | |
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type | Producing Mab mouse CD49d (VLA-4 a chain) |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:9893044 | Enhancement of activation-induced cell death by fibronectin in murine CD4+ CD8+ thymocytes. Takayama E,Kina T,Katsura Y,Tadakuma T Immunology. 1998 Dec;95(4):553-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1370 | Cell Name | TAS-5 |
|---|---|---|---|
| LOT No. | 06152010 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 2mM Glutamine,50uM 2-Mercaptoetanol and 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.402-1.64x10^5 cells/ml | Methods for Passages | split 1/20 (1/10-1/20) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.71x10^6 |
| Viability at cell freezing (%) | 88.8 | Antibiotics Used | free |
| Passage Number | p2* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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