JCRB9092 7D4
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9092 | Cell Name | 7D4 |
|---|---|---|---|
| Profile | anti-murine IL-2 receptors (rat antibody) | Other Name | |
| Animal | mouse x rat | Strain | |
| Genus | Species | ||
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | antibody-producing hybridoma | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | hybridoma, anti-murine IL-2 receptors | |
| Life Span | Crisis PDL | ||
| Morphology | Character | hybridoma, anti-murine IL-2 receptors | |
| Classify | Established by | ||
| Registered by | Regulation for Distribution | ||
| Comment | Prepared from CRL1698. DMEM w/ 4.5g/L glucose, 0.1 mM non essential amino acids, 1 mM sodium pyruvate, 300 micro g/ml L-glutamine. | Year | 1986 |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal calf serum. | Methods for Passages | Dilution. Maintain cultures at cell concentration between 10^5 and 10^6 per ml. |
| Cell Number on Passage | 1-2 x 10^5/ml | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | hybridoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1702762 | Induction of interleukin-1 and -6 in human gingival fibroblast cultures stimulated with Bacteroides lipopolysaccharides. Takada H,Mihara J,Morisaki I,Hamada S Infect Immun. 1991 Jan;59(1):295-301 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9092 | Cell Name | 7D4 |
|---|---|---|---|
| LOT No. | 021089 | Lot Specification | distribution |
| Medium | DMEM ( see comments ) + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 1-2 x 10^5/ml | Methods for Passages | Dilution. Maintain cultures at cell concentration between 10^5 and 10^6 per ml. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.7x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | *P4 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |