JCRB1316.6 OHP7D7.2.3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1316.6 Cell Name   OHP7D7.2.3
Profile   Mouse hybridoma cell line producing monoclonal antibody against 17alpha-hydroxyprogesterone. Other Name   
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgG k.
Classify   hybridoma Established by   Sawada, J.
Registered by   Sawada, J. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab 17alpha-hydroxyprogesterone.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J486
Reference
Pubmed id:1922102Molecular characterization of monoclonal anti-steroid antibodies: primary structures of the variable regions of seven antibodies specific for 17 alpha-hydroxyprogesterone or 11-deoxycortisol and their pH-reactivity profiles.
Sawada J,Mizusawa S,Terao T,Naito M,Kurosawa Y
Mol Immunol. 1991 Oct;28(10):1063-72
Pubmed id:3312820Production and characterization of monoclonal antibodies to 17 alpha-hydroxyprogesterone.
Sawada J,Terao T,Itoh S,Maeda M,Tsuji A,Hosoda H,Nambara T
J Steroid Biochem. 1987 Oct;28(4):405-10

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1316.6 Cell Name   OHP7D7.2.3
LOT No.   1208209 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.303-1.22x10^5 cells/ml Methods for Passages   split 1/5(1/2-1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.97x10^6
Viability at cell freezing (%)   75.9 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1316.6 Cell Name   
LOT No.   12082009 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum (SIGMA 027K03911) Temperature   37 C
Cell Density at Seeding   3 - 4 x 10^4 cells/ml Methods for Passages   Simple dilution, split 1/5 (1/2-1/10)
Doubling Time    Cell Number in Vial (cells/1ml)   4.0 x 10^6
Viability at cell freezing (%)   75.9 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   Cell Banker BLC-1 (Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
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