JCRB1353 TER-119

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1353 Cell Name   TER-119
Profile   Hybridoma cell line producing antibody against mouse erythrocyte membrane antigen Ly76. Other Name   
Animal   mouse x rat Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing antibody against mouse erythrocyte membrane antigen Ly76 (rat IgG 2b and kappa)
Classify   hybridoma Established by   Kina T.
Registered by   Kina T. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI-1640 Medium(10%FBS, 2mM Glutamine, 50uM 2-ME) Methods for Passages   dilution
Cell Number on Passage   split 1/20 (1/10 - 1/20) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   prod. Mab mouse erythrocyte membrane antigen

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8432
Reference
Pubmed id:10848813The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage.
Kina T,Ikuta K,Takayama E,Wada K,Majumdar AS,Weissman IL,Katsura Y
Br J Haematol. 2000 May;109(2):280-7
Pubmed id:1975515A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells.
Ikuta K,Kina T,MacNeil I,Uchida N,Peault B,Chien YH,Weissman IL
Cell. 1990 Sep 7;62(5):863-74

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1353 Cell Name   TER-119
LOT No.   11252009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911), 2mM Glutamine and 50uM 2-Mercaptoetanol. Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   split 1/20(1/10-1/20)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.66x10^6
Viability at cell freezing (%)   81.9 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1353 Cell Name   TER-119
LOT No.   11252015 Lot Specification   distribution
Medium   RPMI-1640 Medium with 50 uM 2-mercaptoethanol and 10% heat-inactivated fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   0.6 - 1.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). The growth at the initiation of culture will be slow.
Doubling Time   62 hrs Cell Number in Vial (cells/1ml)   1.4 x 10^6
Viability at cell freezing (%)   55.7 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   55.7 Additional information   
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