JCRB1350 EL-4

Cell information

Important Notice(s)

EL-4 and EL-4ad: Culture medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1350 Cell Name   EL-4
Profile   EL-4 was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. Other Name   
Animal   mouse Strain   C57BL/6N
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Cohn M
Registered by   Ota, T. Regulation for Distribution   
Comment    Year   2008
Medium   Hams F10:L15=3:7 medium with 10% heat-inactivated fetal bovine serum. (Current medium for propagation: RPMI-1640 medium with 10% FBS.) Methods for Passages   dilution
Cell Number on Passage   -- Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   T-Lymphoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0255
Reference
Pubmed id:1505120Isolation and characterization of a low metastatic variant from EL-4 mouse T-lymphoma.
Ota T,Tanino M,Kohno H,Funamoto H,Odashima S
Clin Exp Metastasis. 1992 Sep;10(5):297-308
Pubmed id:4112916Immunoglobulin and theta-bearing murine leukemias and lymphomas.
Shevach EM,Stobo JD,Green I
J Immunol. 1972 May;108(5):1146-51
Pubmed id:4119883Serological analysis of cell surface antigens of tumors induced by murine leukemia virus.
Herberman RB
J Natl Cancer Inst. 1972 Jan;48(1):265-71
Pubmed id:13316761Passive immunity in mice against C57BL leukosis E.L. 4 by means of iso-immune serum.
GORER PA,AMOS DB
Cancer Res. 1956 May;16(4):338-43

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1350 Cell Name   EL-4
LOT No.   11132009 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.8-5.5x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   95.9 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1350 Cell Name   EL-4
LOT No.   10262015 Lot Specification   distribution
Medium   RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   5.3 - 5.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 14 hrs. Cell Number in Vial (cells/1ml)   5.7 x 10^5
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI-1640 CO2 Conc.   5%
Viability immediately after thawing (%)   96.6 Additional information   
Cell No.   JCRB1350 Cell Name   EL-4
LOT No.   02052018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 - 5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). This cell line grows very rapidly and should be passaged before reaching to 1.5 x 10^6 cells/mL.
Doubling Time   approx. 12 hrs Cell Number in Vial (cells/1ml)   2.9 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (10 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   93 Additional information   
Cell No.   JCRB1350 Cell Name   EL-4
LOT No.   02172025 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   6 - 7 x 10^4 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 13 hrs Cell Number in Vial (cells/1ml)   5.9 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   73 Additional information   
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