JCRB1348 3LL

Cell information

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Lewis lung carcinoma (3LL): Culture medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1348 Cell Name   3LL
Profile   Lewis lung carcinoma cell line. Other Name   LLC
Animal   mouse Strain   
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   lung
Case history   Lewis lung carcinoma Metastasis   Yes
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Cell line having high metastasis potencial to lung.
Classify   tumor Established by   Sugimoto, Y.
Registered by   Ota, T. Regulation for Distribution   
Comment    Year   2008
Medium   Hams F10:L15=3:7 medium with 10% heat-inactivated fetal bovine serum. (Current medium for propagation: RPMI-1640 medium with 10% FBS) Methods for Passages   Cells harvested after treatment with PBS containing 0.125% trypsin and 1 mM EDTA. Change medium twice a week.
Cell Number on Passage   -- Race   
CO2 Conc.   5% Tissue Sampling   Lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5653
Reference
Pubmed id:11299736Functional suppression of integrin beta 4-mediated adhesion caused by in vivo sequential selection for cancer cell intravasation.
Ota T,Maeda M,Tanino M,Tatsuka M
Anticancer Res. 2001 Jan-Feb;21(1A):205-11
Pubmed id:10408901Decrease of metastatic ability after selection for intravasating ability in Lewis lung carcinoma (3LL) cell line.
Ota T,Maeda M,Tatsuka M,Matsui T,Tanino M,Tanaka T
Cancer Lett. 1999 May 3;139(1):105-8
Pubmed id:7226139Establishment of a cloned line of Lewis Lung Carcinoma cells adapted to cell culture.
Bertram JS,Janik P
Cancer Lett. 1980 Nov;11(1):63-73

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1348 Cell Name   3LL
LOT No.   10202009 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were harvested after treatment with 0.125% trypsin and 1mM EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.57x10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1348 Cell Name   3LL
LOT No.   04122013 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated fetal bovine serum. (FBS; GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.8 - 1.7 x 10^5 cells / mL Methods for Passages   Cells harvested after treatment with PBS containing 0.125% trypsin and 1 mM EDTA. Change medium twice a week.
Doubling Time    Cell Number in Vial (cells/1ml)   6.7 x 10^6 cells / m
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   10% DMSO, 20% FBS, RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1348 Cell Name   3LL
LOT No.   07112016 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.5 - 2.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.125% trypsin and 0.02% EDTA.
Doubling Time   approx. 2 days Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   88.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   88.0 Additional information   
Cell No.   JCRB1348 Cell Name   3LL
LOT No.   08232018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.2 - 2.3 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.125% trypsin and 0.02% EDTA.
Doubling Time   approx. 33 hrs Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   82 Additional information   
Cell No.   JCRB1348 Cell Name   3LL
LOT No.   04242024 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 1.5 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.125% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   75.2 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   75.2 Additional information   
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