JCRB9089 CCRF S-180 II

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9089 Cell Name   CCRF S-180 II
Profile   S-180 sarcoma derivative Other Name   sarcoma 180
Animal   mouse Strain   CFW
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   sarcoma 180 derived
Life Span    Crisis PDL   
Morphology    Character   sarcoma 180
Classify   tumor Established by   Foley et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 8. Year   1985
Medium   Eagle's minimal essential medium with non-essential amino acids and 5% calf serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/2 - 1/6 Race   
CO2 Conc.   5 % Tissue Sampling   sarcoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2874
Reference
Pubmed id:14185313ANIMAL CELL STRAINS. THE CELL CULTURE COLLECTION COMMITTEE HAS ASSEMBLED AND CERTIFIED 23 STRAINS OF ANIMAL CELLS.
LEDLEY RS
Science. 1964 Oct 9;146(3641):241-3
Pubmed id:13823721Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines.
FOLEY GE,DROLET BP,McCARTHY RE,GOULET KA,DOKOS JM,FILLER DA
Cancer Res. 1960 Jul;20():930-9
Pubmed id:13350343Sustained propagation of sarcoma of 180 in tissue culture.
FOLEY GE,DROLET BP
Proc Soc Exp Biol Med. 1956 Jun;92(2):347-52




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9089 Cell Name   
LOT No.   07312007 Lot Specification   distribution
Medium   Eagle's minimam essential medium with non-essential amino acids and 5% calf serum Temperature   37 C
Cell Density at Seeding   4 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   approx. 19 hrs Cell Number in Vial (cells/1ml)   3.5 x 10^6
Viability at cell freezing (%)   96.0 Antibiotics Used   free
Passage Number   P97 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMEO, 20% CS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9089 Cell Name   
LOT No.   041399 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essenti-al amino acids and 5% calf serum Temperature   37 C
Cell Density at Seeding   7-8 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time   D.T.=ca 16 hrs Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P97 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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