JCRB1344 delta-47
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1344 | Cell Name | delta-47 |
|---|---|---|---|
| Profile | Human myeloma cell line secreting IgD. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 72 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | left testicular tumor | Metastasis | |
| Tissue Metastasized | Genetics | The cell line expresses CD4 & CD38. Aneuploid karyotype including 1q+, 2p+ & 14q+. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | plasmacytoid | Character | Cells are secreting IgD. |
| Classify | tumor | Established by | Kubonishi,I. |
| Registered by | Miyoshi,I. | Regulation for Distribution | |
| Comment | Year | 2007 | |
| Medium | RPMI 1640 medium with 20% fetal calf serum. | Methods for Passages | Dilution |
| Cell Number on Passage | Split ratio = 1/2 - 1/3. | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | ascites |
| Tissue Type | myeloma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1329502 | IgD myeloma presenting as a testicular tumor: establishment and characterization of an IgD-secreting myeloma cell line. Ishii K,Yamato K,Kubonishi I,Taguchi H,Ohtsuki Y,Miyoshi I Am J Hematol. 1992 Nov;41(3):218-24 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1344 | Cell Name | |
|---|---|---|---|
| LOT No. | 08022010 | Lot Specification | distribution |
| Medium | RPMI1640 medium with 10% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099) | Temperature | 37 C |
| Cell Density at Seeding | 1.0 - 1.2 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture), split ratio = 1/2 - 1/3. |
| Doubling Time | approx. 50 hrs | Cell Number in Vial (cells/1ml) | 3.2 x 10^6 |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | Unknown (7 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB1344 | Cell Name | delta-47 |
|---|---|---|---|
| LOT No. | 07212009 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 4.3x10^5 cells/ml | Methods for Passages | split ratio=1/2-1/3 |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.5x10^6 |
| Viability at cell freezing (%) | 79.5 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:8 D7S820:10 D16S539:9,11 VWA:14,20 TH01:9 AM:X TPOX:8,10 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1344 | Cell Name | delta-47 |
|---|---|---|---|
| LOT No. | 10282019 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37C |
| Cell Density at Seeding | approx. 2.1 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture). |
| Doubling Time | approx. 2 days | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 |
| Viability at cell freezing (%) | 90.0 | Antibiotics Used | free |
| Passage Number | Unknown (8 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 81 | Additional information |