JCRB9087 RPMI 1846

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9087 Cell Name   RPMI 1846
Profile   melanotic melanoma Other Name   RPMI 1846
Animal   hamster, Syrian Strain   
Genus   Mesocricetus Species   auratus
Sex    Age   
Identity    Tissue for Primary Cancer   melanoma
Case history    Metastasis   
Tissue Metastasized    Genetics   melanotic melanoma
Life Span    Crisis PDL   
Morphology    Character   melanotic melanoma
Classify    Established by   Moore, G.E. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 49. Year   1985
Medium   McCoy's 5a medium with 20% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 - 1/5 Race   
CO2 Conc.   5 % Tissue Sampling   melanotic melanoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2711
Reference
Pubmed id:4522794A diffusible factor restoring contact inhibition of growth to malignant melanocytes.
Lipkin G,Knecht ME
Proc Natl Acad Sci U S A. 1974 Mar;71(3):849-53
Pubmed id:14071829CULTURE OF MALIGNANT TUMORS OF THE SYRIAN HAMSTER.
MOORE GE,MOUNT D,TARA G,SCHWARTZ N
J Natl Cancer Inst. 1963 Nov;31():1217-37
Pubmed id:13700922Transplantable tumors of the Syrian (golden) hamster. I. Tumors of the alimentary tract, endocrine glands and melanomas.
FORTNER JG,MAHY AG,SCHRODT GR
Cancer Res. 1961 Jul;21(6)Pt 2():161-98
Pubmed id:14403619Primary cultivation and continuous propagation in vitro of tissues from small biopsy specimens.
HSU TC,KELLOGG DS Jr
J Natl Cancer Inst. 1960 Aug;25():221-35




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9087 Cell Name   RPMI 1846
LOT No.   092987 Lot Specification   distribution
Medium   McCoy's 5a medium + FCS 20% Temperature   37
Cell Density at Seeding   1/2 - 1/5 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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