JCRB1337 DL-40

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1337 Cell Name   DL-40
Profile   Ki-1-positive T-cell lymphoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   64
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   leukemic phase of non-Hodgkin's lymphoma Metastasis   
Tissue Metastasized    Genetics   Cells are positive for acid phosphatase and negative for peroxidase, EBNA and HTLV-1 antigen.
Life Span   infinite Crisis PDL   
Morphology   lymphoid Character   Cells grow without forming clumps with Dt=72 hrs.
Classify   tumor Established by   Kubonishi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   Dilution.
Cell Number on Passage   Split ratio = 1/2 -1/3. Race   Japanese
CO2 Conc.   5% Tissue Sampling   peripheral blood
Tissue Type   lymphoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2889
Reference
Pubmed id:1979248A Ki-1 (CD30)-positive T (E+, CD4+, Ia+)-cell line, DL-40, established from aggressive large cell lymphoma.
Kubonishi I,Sonobe H,Miyagi T,Iwahara Y,Ohyashiki JH,Ohyashiki K,Toyama K,Ohtsuki Y,Miyoshi I
Cancer Res. 1990 Dec 1;50(23):7682-5

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1337 Cell Name   DL-40
LOT No.   10262010 Lot Specification   distribution
Medium   RPMI 1640 medium with 10 - 20% heat-inactivated fetal bovine serum (GIBCO Cat. # 10099). Use 20% FBS up to the cells stably grow. Temperature   37 C
Cell Density at Seeding   approx. 3.0 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture)
Doubling Time   27 - 98 hrs. Cell Number in Vial (cells/1ml)   8.6 x 10^5
Viability at cell freezing (%)   78.2 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   Yes
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1337 Cell Name   DL-40
LOT No.   05152009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10-20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   7.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   70.9 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,12
D13S317:9,13
D7S820:10,11
D16S539:10,11
VWA:14,19
TH01:8
AM:X
TPOX:8,9
CSF1PO:13,14
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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