JCRB1334 RC-K8

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1334 Cell Name   RC-K8
Profile   A human lymphoma cell line with t(11;14) chromosome abnormality. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   55
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   malignant lymphoma (histiocytic lymphoma) Metastasis   
Tissue Metastasized    Genetics   46XY, with t(11;14)(q23;q32).
Life Span   infinite Crisis PDL   
Morphology   lymphoid Character   Dt=48-60hr. The cell line produces plasminogen activator.
Classify   tumor Established by   Kubonishi,I.
Registered by   Miyoshi,I. Regulation for Distribution   Distribution to commercial companies should be informed to Dr. Miyoshi.
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage   1/2 - 1/3 Race   Japanese
CO2 Conc.   5% Tissue Sampling   chylous peritoneal effusion
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1883
Reference
Pubmed id:3742463Characterization of a new human lymphoma cell line (RC-K8) with t(11;14) chromosome abnormality.
Kubonishi I,Niiya K,Yamashita M,Yano S,Abe T,Ohtsuki Y,Miyoshi I
Cancer. 1986 Oct 1;58(7):1453-60

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1334 Cell Name   RC-K8
LOT No.   04202009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   dilution. Split ratio=1/2-1/3
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.13x10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,11
D13S317:9
D7S820:10.3,12
D16S539:10
VWA:18,19
TH01:7
AM:X,Y
TPOX:8
CSF1PO:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1334 Cell Name   RC-K8
LOT No.   07052016 Lot Specification   distribution
Medium   RPMI-1640 medium with 20% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 1702012). Temperature   37 C
Cell Density at Seeding   2.5 - 9.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 82 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   87.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   87.0 Additional information   
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