JCRB9080 C3H/10T1/2 clone 8

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9080 Cell Name   C3H/10T1/2 clone 8
Profile   fibroblast, contact inhibited, (C3H mouse) Other Name   
Animal   mouse Strain   C3H
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   embryo, contact sensitive
Life Span    Crisis PDL   
Morphology    Character   embryo, contact sensitive
Classify    Established by   Reznikoff,C et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL226. Year   1985
Medium   Basal medium Eagle with 10% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.15% trypsin.
Cell Number on Passage   2 x 10^3 cells/cm^2 Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0190
Reference
Pubmed id:1277196In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells.
Jones PA,Laug WE,Gardner A,Nye CA,Fink LM,Benedict WF
Cancer Res. 1976 Aug;36(8):2863-7
Pubmed id:129280Oncogenic transformation of C3H/10T1/2 clone 8 mouse embryo cells by halogenated pyrimidine nucleosides.
Jones PA,Benedict WF,Baker MS,Mondal S,Rapp U,Heidelberger C
Cancer Res. 1976 Jan;36(1):101-7
Pubmed id:1167940Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation.
Terzaghi M,Little JB
Nature. 1975 Feb 13;253(5492):548-9
Pubmed id:4812259Cell cycle dependency of oncogenic transformation induced by N-methyl-N'-nitro-N-nitrosoquanidine in culture.
Bertram JS,Heidelberger C
Cancer Res. 1974 Mar;34(3):526-37
Pubmed id:4796800Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.
Reznikoff CA,Bertram JS,Brankow DW,Heidelberger C
Cancer Res. 1973 Dec;33(12):3239-49




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9080 Cell Name   
LOT No.   04122005 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. Do not allow the culture to confluence for long period.
Doubling Time   approx. 30 hrs Cell Number in Vial (cells/1ml)   5.1 x 10^5
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   P11 after cloning PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as mouse by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9080 Cell Name   C3H/10T1/2 clone 8
LOT No.   100793 Lot Specification   distribution
Medium   Basal medium of Eagle(GIBCO) with Earle's salts with 10% fetal bovine serum (heat inactivated) Temperature   37 C
Cell Density at Seeding   1.3x10^5 cells/10cm dish Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.86x10^6
Viability at cell freezing (%)   91.9 Antibiotics Used   free
Passage Number   P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9080 Cell Name   C3H/10T1/2 clone 8
LOT No.   021491 Lot Specification   distribution
Medium   EMEM(lot.12591) + FBS(10%)(J.R. Co.19407) Temperature   37
Cell Density at Seeding   1.4x10^5 cells/10 cm dish Methods for Passages   0.02% EDTA + 0.2 % trypsin
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.02x10^6
Viability at cell freezing (%)   93.1 Antibiotics Used   free
Passage Number   P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9080 Cell Name   C3H/10T1/2 clone 8
LOT No.   03242020 Lot Specification   distribution
Medium   Basal medium Eagle with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2 - 3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   5.3 x 10^5
Viability at cell freezing (%)   99.3 Antibiotics Used   free
Passage Number   P12 after cloning PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - BME CO2 Conc.   5%
Viability immediately after thawing (%)   91.4 Additional information   
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