JCRB9077 MMT 060562
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9077 | Cell Name | MMT 060562 |
|---|---|---|---|
| Profile | spontaneous mammary tumor | Other Name | MMT |
| Animal | mouse | Strain | |
| Genus | Mus | Species | musculus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | mammary | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | spontaneous mammary tumor | |
| Life Span | Crisis PDL | ||
| Morphology | Character | spontaneous mammary tumor | |
| Classify | Established by | Sykes, J. | |
| Registered by | Regulation for Distribution | Following rules from ATCC | |
| Comment | Prepared from CCL 51. | Year | 1986 |
| Medium | MEM with NEAA + CS 10% | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin |
| Cell Number on Passage | 1/2 - 1/5 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | mammary tumor |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9077 | Cell Name | MMT 060562 |
|---|---|---|---|
| LOT No. | 11262001 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 4 x 10^4 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS |
| Doubling Time | Cell Number in Vial (cells/1ml) | 2.1 x 10^6 | |
| Viability at cell freezing (%) | 91.3 | Antibiotics Used | free |
| Passage Number | P207 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | 10% DMSO, 20% FBS-base medium | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Additional information | mislabeled as MMT060502. |
| Cell No. | JCRB9077 | Cell Name | MMT 060562 |
|---|---|---|---|
| LOT No. | 092590 | Lot Specification | distribution |
| Medium | MEM w/ non essential amino acids + CS 10% | Temperature | 37 |
| Cell Density at Seeding | 1/2 - 1/5 | Methods for Passages | 0.02% EDTA and 0.25% trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.8x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P207 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |