JCRB9075 NCTC Clone 1469
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9075 | Cell Name | NCTC Clone 1469 |
|---|---|---|---|
| Profile | Normal cell line from mouse liver. | Other Name | NCTC Clone 1469 |
| Animal | mouse | Strain | C3H/An |
| Genus | Mus | Species | musculus |
| Sex | M | Age | |
| Identity | Tissue for Primary Cancer | liver | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | liver | |
| Life Span | Crisis PDL | ||
| Morphology | epithelial-like | Character | liver |
| Classify | normal | Established by | Hobbs, G.L., et al. |
| Registered by | Purchased from ATCC | Regulation for Distribution | Following rules from ATCC |
| Comment | CCL 9.1. | Year | 1985 |
| Medium | NCTC 135 medum with horse serum 10% | Methods for Passages | Scraping. |
| Cell Number on Passage | 1/2 - 1/4 | Race | |
| CO2 Conc. | 5 % | Tissue Sampling | liver |
| Tissue Type | normal |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:13766009 | A hepatitis virus complicating studies with mouse leukemia. MANAKER RA,PICZAK CV,MILLER AA,STANTON MF J Natl Cancer Inst. 1961 Jul;27():29-51 |
| Pubmed id:14431674 | Glycogen content of cell suspensions prepared from massive tissue culture. II. Comparison of fourteen cell strains after long cultivation. PEPPERS EV,WESTFALL BB,EARLE WR J Natl Cancer Inst. 1959 Oct;23():823-31 |
| Pubmed id:13619759 | Long-term cultivation of animal tissue cells in large cultures. EARLE WR Fed Proc. 1958 Dec;17(4):967-74 |
| Pubmed id:13523589 | Studies on culture lines derived from mouse liver parenchymatous cells grown in long-term tissue culture. EVANS VJ,HAWKINS NM,WESTFALL BB,EARLE WR Cancer Res. 1958 Apr;18(3):261-6 |
| Pubmed id:13502690 | Establishment of a clone of mouse liver cells from a single isolated cell. HOBBS GL,SANFORD KK,EVANS VJ,EARLE WR J Natl Cancer Inst. 1957 May;18(5):701-7 |
| Pubmed id:14939026 | The growth in vitro of massive cultures of liver cells. EVANS VJ,EARLE WR,WILSON EP,WALTZ HK,MACKEY CJ J Natl Cancer Inst. 1952 Jun;12(6):1245-65 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9075 | Cell Name | NCTC Clone 1469 |
|---|---|---|---|
| LOT No. | 081092 | Lot Specification | distribution |
| Medium | NCTC135(Flow Laboratory lot#2010222) + horse serum 10% (J.R.Scientific lot#OD1223) | Temperature | 37C |
| Cell Density at Seeding | 1x10^6 | Methods for Passages | scraping (floating cells were also cultivated at the same time.) |
| Doubling Time | 12Hrs. | Cell Number in Vial (cells/1ml) | 2.16x10^6 |
| Viability at cell freezing (%) | 97% | Antibiotics Used | free |
| Passage Number | P21 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LD,NP |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9075 | Cell Name | |
|---|---|---|---|
| LOT No. | 022398 | Lot Specification | distribution |
| Medium | NCTC 135 medium with 10% horse serum | Temperature | 37 C |
| Cell Density at Seeding | 1 x 10^5 cells/ml | Methods for Passages | Scraping, or 0.25% trypsin and 0.02% EDTA |
| Doubling Time | ca. 48 hrs | Cell Number in Vial (cells/1ml) | 4.2 x 10^6 |
| Viability at cell freezing (%) | 92.0 | Antibiotics Used | free |
| Passage Number | P19 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as mouse by NP, G6PD, LD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |