JCRB1222.03 HGH1.17.2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1222.03 Cell Name   HGH1.17.2
Profile   Mouse hybridoma cell line producing monoclonal antibody against human growth hormone (hGH). Other Name   
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgG gamma1 and k
Classify   hybridoma Established by   Sawada, J.
Registered by   Sawada, J. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab human growth hormone (hGH).

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J456
Reference
Pubmed id:2427940Comparison of the antigenicities of native human growth hormone (hGH) and three forms of recombinant hGHs using monoclonal antibodies.
Sawada J,Wada N,Irie M,Tokunaga-Doi T,Ohtsuka E,Ikehara M,Terao T
Mol Immunol. 1986 Jun;23(6):625-30

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1222.03 Cell Name   HGH1.17.2
LOT No.   08042008 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   3.33-9.20x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.1x10^6
Viability at cell freezing (%)   79.7 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)   73 Additional information   
Images
Cell No.   JCRB1222.03 Cell Name   HGH1.17.2
LOT No.   11112020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO:catalog No.11875-093) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   2.31-3.26x10^4 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.98x10^6
Viability at cell freezing (%)   81 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker (Nihon Zenyaku Kogyo Co.Ltd:catalog No.CB011) CO2 Conc.   5%
Viability immediately after thawing (%)   45.1 Additional information   
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