JCRB1314 HPSV178-4-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1314 Cell Name   HPSV178-4-3
Profile   Hybridoma cell line producing mAb HPSV178, against the C-terminal portion of PrPSV (residues 214-230). Other Name   
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   No
Tissue Metastasized    Genetics   Producing IgG2b(K)
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   producing mAb HPSV178, against the C-terminal portion of PrPSV (residues 214-230).
Classify   hybridoma Established by   Kikuchi, Y.
Registered by   Kikuchi, Y. Regulation for Distribution   
Comment    Year   2008
Medium   RPMI1640 medium with 10% fetal bovine serum (100 uM Glutamine, 1 mM Sodium pyruvate and 50 uM 2-mercaptoetanol). Methods for Passages   dilution
Cell Number on Passage   split 1/10 - 1/20 Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J292
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1314 Cell Name   HPSV178-4-3
LOT No.   07172008 Lot Specification   distribution
Medium   RPMI1640 medium with 50uM 2-Mercaptoetanol ,1mM Sodium pyruvate and 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.1c10^5 cells/ml Methods for Passages   1/10-1/20 dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.4x10^6
Viability at cell freezing (%)   85.1 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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