JCRB1313 Ra-1

Cell information

Cell type：virus production cells (View Pricing Information)

JCRB No.   JCRB1313 Cell Name   Ra-1
Profile   Rabbit lymphocyte cell line transformed with HTLV-1. Other Name   
Animal   rabbit Strain   
Genus   Oryctolagus Species   cuniculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   hemo-lymphocytic
Case history   Rabbit cells were cocultivated with MT-2 cells. Metastasis   
Tissue Metastasized    Genetics   HTLV-1 producing cell line.
Life Span   infinite Crisis PDL   
Morphology   lymphoid Character   
Classify   transformed Established by   Miyoshi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage   Split ratio = 1/2 -1/3. Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   lymphocyte

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2698
Reference
Pubmed id:6301915Transformation of rabbit lymphocytes with T-cell leukemia virus.
Miyoshi I,Yoshimoto S,Taguchi H,Kubonishi I,Fujishita M,Ohtsuki Y,Shiraishi Y,Akagi T
Gan. 1983 Feb;74(1):1-4

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1313 Cell Name   Ra-1
LOT No.   07102008 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO catalog No.11875) with 15% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   4.0x10^5 cells/ml Methods for Passages   dilution,split ratio 1/2-1/3
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.8x10^6
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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