JCRB9071 Daudi

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9071 Cell Name   Daudi
Profile   lymphoma, Burkitt's Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   16
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   Burkitt lymphoma
Life Span    Crisis PDL   
Morphology    Character   Burkitt's lymphoma
Classify    Established by   Klein,E. & Klein,G.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL213. Year   1987
Medium   RPMI1640 medium with 20% fetal calf serum Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml .
Cell Number on Passage   5-7 x 10^5/ml Race   
CO2 Conc.   5 % Tissue Sampling   Burkitt lymphoma
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0008
Reference
Pubmed id:201572Chromosome banding, isoenzyme studies and determination of Epstein-Barr virus DNA content on human Burkitt lymphoma/mouse hybrids.
Spira J,Povey S,Wiener F,Klein G,Andersson-Anvret M
Int J Cancer. 1977 Dec 15;20(6):849-53

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   01292009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 20% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   0.6-1.3x10^5 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.24x10^6
Viability at cell freezing (%)   99.8 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8,13
D13S317:11,12
D7S820:8,10
D16S539:10,12
VWA:15,17
TH01:6,7
AM:X,Y
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9071 Cell Name   
LOT No.   09262003 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (FBS lot; GIBCO 3762782S) Temperature   37 C
Cell Density at Seeding   2 x 10^5 cells/ml Methods for Passages   Dilution with subcultivation interval 2-3 days.
Doubling Time   D.T. = 29 hrs Cell Number in Vial (cells/1ml)   2.1 x 10^6
Viability at cell freezing (%)   92.4 Antibiotics Used   free
Passage Number   P6* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST, LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   051796 Lot Specification   distribution
Medium    Temperature   
Cell Density at Seeding    Methods for Passages   
Doubling Time    Cell Number in Vial (cells/1ml)   
Viability at cell freezing (%)    Antibiotics Used   
Passage Number    PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   120694 Lot Specification   distribution
Medium   RPMI1640 medium with 20% heat inactivated fetal bovine serum (Hyclone, 11151031) Temperature   37 C
Cell Density at Seeding   3-4x10^5 cells/ml Methods for Passages   Simple dilution.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.1x10^6
Viability at cell freezing (%)   87.9 Antibiotics Used   free
Passage Number   P6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   LDH, NP
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   100992 Lot Specification   distribution
Medium   RPMI1640 medium (081792) with 20% heat inactivatedfetal bovine serum (JRH Bioscience 1B1062) Temperature   37 C
Cell Density at Seeding   1.7x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   *P4 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   022791 Lot Specification   distribution
Medium   RPMI1640 + FCS 20% Temperature   37 C
Cell Density at Seeding   5-7 x 10^5/ml Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P6 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   M=46 Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   021288 Lot Specification   distribution
Medium   RPMI1640 + FCS 20% Temperature   37
Cell Density at Seeding   5-7 x 10^5/ml Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P4* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   04242013 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal calf serum (FBS: GIBCO Cat. #10099) Temperature   37 C
Cell Density at Seeding   2.7 - 6.5 x 10^5/ml Methods for Passages   Dilution. A maximum of 2-3 x 10^6/ml .
Doubling Time    Cell Number in Vial (cells/1ml)   6.8 x 10^5 cells/mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   86.1 Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   09062017 Lot Specification   distribution
Medium   RPMI1640 medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.4 - 1.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 22 hrs. Cell Number in Vial (cells/1ml)   1.7 x 10^6
Viability at cell freezing (%)   83.0 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83.0 Additional information   
Cell No.   JCRB9071 Cell Name   Daudi
LOT No.   10052022 Lot Specification   distribution
Medium   RPMI 1640 medium with 20% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   2.0 - 5.3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   6.6 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:8,13
D13S317:11,12
D7S820:8,10
D16S539:10,12
VWA:15,17
TH01:6,7
AM:X,Y
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
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