JCRB9069 SW-13

Cell information

Important Notice(s)

Leibovitz's L-15 medium

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9069 Cell Name   SW-13
Profile   Human adenocarcinoma derived from adrenal cortex. Other Name   SW-13
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   adrenal cortex
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   adenocarcinoma, Adrenal cortex
Classify   tumor Established by   Leibovitz,A. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL105. Year   1987
Medium   Leibovitz's L-15 medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   1/3 - 1/10 Race   
CO2 Conc.   - Tissue Sampling   adrenal cortex
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0542
Reference
Pubmed id:841332Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:4765382New human cancer cell culture lines. I. SW-13, small-cell carcinoma of the adrenal cortex.
Leibovitz A,McCombs WM 3rd,Johnston D,McCoy CE,Stinson JC
J Natl Cancer Inst. 1973 Aug;51(2):691-7
Pubmed id:4339896Gap junctions in normal and transformed fibroblasts in culture.
Pinto da Silva P,Gilula NB
Exp Cell Res. 1972;71(2):393-401
Pubmed id:13611537Cultivation of human breast carcinomas.
LASFARGUES EY,OZZELLO L
J Natl Cancer Inst. 1958 Dec;21(6):1131-47

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9069 Cell Name   
LOT No.   05252009 Lot Specification   distribution
Medium   Leibovitz's L-15 medium with 10% fetal bovine serum. This medium is intended to be used at normal atmosphere (not in 5% CO2). If you use CO2 incubator, seal the flask cap tightly. Temperature   37 C
Cell Density at Seeding   approx. 5 x 10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   94.0 Antibiotics Used   free
Passage Number   P26 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - L15 medium CO2 Conc.   No
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9069 Cell Name   SW-13
LOT No.   071895 Lot Specification   distribution
Medium   Leibovitz's L-15 medium with 10% fetal bovine serum (J.R.H.Biosciences, 1B1062) Temperature   37 C
Cell Density at Seeding   6.7x10^5 cells/100mm dish Methods for Passages   Wash cells once with PBS & treated with 0.02% EDTA& 0.25% trypsin at 37C for 15min.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.4x10^6
Viability at cell freezing (%)   72.0 Antibiotics Used   free
Passage Number   P27 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   0 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9069 Cell Name   
LOT No.   F00992 Lot Specification   distribution
Medium   L-15 (leibovitz's) medium with 10% FCS Temperature   37
Cell Density at Seeding   1/3-1/10 Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   7.1 x 10^6
Viability at cell freezing (%)   90.0 Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by isoenzymology (NP,G6PD,AST)
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9069 Cell Name   SW-13
LOT No.   031389 Lot Specification   distribution
Medium   L-15(Leibovitz's) medium + 10% FBS Temperature   37
Cell Density at Seeding   1/3 - 1/10 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P25 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   0 %
Viability immediately after thawing (%)    Additional information   
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