JCRB1225 I51T

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1225 Cell Name   I51T
Profile   Mouse behta-galactosidase deficient cell line introduced human defcient bethta-galactosidase gene. Other Name   
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musculus
Sex    Age   4 day-old
Identity    Tissue for Primary Cancer   skin
Case history   The mouse SV cell line introduced deficient behta-galactosidase gene(I51T). Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Namba,E. and Matsuda,J.
Registered by   Higaki,K. Regulation for Distribution   
Comment   Cells were originally contaminated by mycoplasmas and decontaminated in the JCRB Cell Bank. Year   2005
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum. 37 C. Methods for Passages   Cells are harvested after treatment with standard trypsin-EDTA.
Cell Number on Passage   Split ratio=1/3-1/4. Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5530
Reference
Pubmed id:14676316Chemical chaperone therapy for brain pathology in G(M1)-gangliosidosis.
Matsuda J,Suzuki O,Oshima A,Yamamoto Y,Noguchi A,Takimoto K,Itoh M,Matsuzaki Y,Yasuda Y,Ogawa S,Sakata Y,Nanba E,Higaki K,Ogawa Y,Tominaga L,Ohno K,Iwasaki H,Watanabe H,Brady RO,Suzuki Y
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15912-7
Pubmed id:11504597Galactonojirimycin derivatives restore mutant human beta-galactosidase activities expressed in fibroblasts from enzyme-deficient knockout mouse.
Tominaga L,Ogawa Y,Taniguchi M,Ohno K,Matsuda J,Oshima A,Suzuki Y,Nanba E
Brain Dev. 2001 Aug;23(5):284-7
Pubmed id:9337086Beta-galactosidase-deficient mouse as an animal model for GM1-gangliosidosis.
Matsuda J,Suzuki O,Oshima A,Ogura A,Noguchi Y,Yamamoto Y,Asano T,Takimoto K,Sukegawa K,Suzuki Y,Naiki M
Glycoconj J. 1997 Sep;14(6):729-36

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1225 Cell Name   I51T
LOT No.   01252008 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(Intergen Lot.RB52305) Temperature   37 C
Cell Density at Seeding   4.7x10^5 cells/dish Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio=1/3-1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   NT
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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