JCRB9067 NRK-49F

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9067 Cell Name   NRK-49F
Profile   fibroblastic clone of NRK (normal rat kidney) cells, useful for transformation assays, (Osbone-Mendel rat) Other Name   
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   kidney
Case history    Metastasis   
Tissue Metastasized    Genetics   normal kidney fibroblast
Life Span    Crisis PDL   
Morphology    Character   normal kidney fibroblast
Classify    Established by   DeLarco,J.E.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL1570. Year   1987
Medium   DMEM(glucose 4.5g/l),non essen amino acids + CS 5% Methods for Passages   0.02% EDTA and 0.25% trypsin. Never allow cultures to become completely confluent.
Cell Number on Passage   split ratio=1/3 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2144
Reference
Pubmed id:2219594Transforming growth factor beta-like activity in human hydrocele fluid.
Saha PK,Kanda S,Morimitsu H,Nishimura N,Kanetake H,Saito Y
Urol Res. 1990;18(4):295-8
Pubmed id:3137084Isolation of 110K actin binding protein from mammalian brain and its immunocytochemical localization within cultured cells.
Maekawa S,Sakai H
Exp Cell Res. 1988 Sep;178(1):127-42
Pubmed id:304450Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.
de Larco JE,Todaro GJ
J Cell Physiol. 1978 Mar;94(3):335-42




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9067 Cell Name   NRK-49F
LOT No.   021293 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with Non Essential Amino Acids and 4.5g/l glucose and 5% CS. Temperature   37 C
Cell Density at Seeding   7.2 x 10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA. Harvest and passage at semiconfluent stage.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1x10^6
Viability at cell freezing (%)   95.2 Antibiotics Used   free
Passage Number   P24 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9067 Cell Name   NRK-49F
LOT No.   120691 Lot Specification   distribution
Medium   DMEM (High glucose concentration) + CS 5% Temperature   37 C
Cell Density at Seeding   5.3x10^5/m Methods for Passages   0.02 % EDTA and 0.2 % trypsin treatment
Doubling Time    Cell Number in Vial (cells/1ml)   1.6x10^6
Viability at cell freezing (%)   99.% Antibiotics Used   free
Passage Number   P24 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9067 Cell Name   NRK-49F
LOT No.   062890 Lot Specification   distribution
Medium   DMEM(glucose 4.5g/l),non essen amino acids + CS 5% Temperature   37 C
Cell Density at Seeding   1/3 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9067 Cell Name   NRK-49F
LOT No.   031987 Lot Specification   distribution
Medium   DMEM(glucose 4.5g/l),non essen amino acids + CS 5% Temperature   37 C
Cell Density at Seeding   1/3 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin. Never allow cultures to become completely confluent.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.7x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P21 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9067 Cell Name   
LOT No.   091997 Lot Specification   distribution
Medium   Dulbecco's MEM with 4.5 g/L glucose, non-essential amino acids and 5% calf serum (or FCS) Temperature   37 C
Cell Density at Seeding   5-6 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   4.4 x 10^5
Viability at cell freezing (%)   78.0 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as rat by NP, G6PD, MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9067 Cell Name   NRK-49F
LOT No.   11052025 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (high glucose) with non-essential amino acids and 5% newborn calf serum (NCS; Biowest Cat. #S0750, Lot S14368S0750) (You can use FBS instead of NCS) Temperature   37 C
Cell Density at Seeding   approx. 8 x 10^3 cells/cm2 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1 day Cell Number in Vial (cells/1ml)   8.6 x 10^5
Viability at cell freezing (%)   97.2 Antibiotics Used   free
Passage Number   P22 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% NCS - basal culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   86.3 Additional information   
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