JCRB1213.3 DIG104.10H.1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1213.3 Cell Name   DIG104.10H.1
Profile   Mouse hybridoma cell line producing monoclonal antibody against digoxin. Other Name   
Animal   mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   antibody-producing hybridoma
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing anti Digoxin IgG1 (k) isotype (RIA 120/130000)
Classify   hybridoma Established by   Sawada,J.
Registered by   Sawada,J. Regulation for Distribution   
Comment    Year   2007
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab digoxin.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J289
Reference
Pubmed id:2089119Determination of cardenolides in hairy root cultures of Digitalis lanata by enzyme-linked immunosorbent assay.
Yoshimatsu K,Satake M,Shimomura K,Sawada JI,Terao T
J Nat Prod. 1990 Nov-Dec;53(6):1498-502
Pubmed id:1364357Production and characterization of monoclonal and polyclonal antibodies against digoxin.
Sawada J,Janejai N,Terao T
Eisei Shikenjo Hokoku. 1990;(108):29-33

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1213.3 Cell Name   DIG104.10H.1
LOT No.   11052007 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.0x10^5 cells/ml Methods for Passages   dilution 1/5 (1/2-1/10)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   76.7 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1213.3 Cell Name   DIG104.10H.1
LOT No.   11052018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 1 x 10^5 cells/mL Methods for Passages   Simple dilution. Saturation cell density will be 1.3 x 10^6 cells/mL.
Doubling Time   approx. 20 hrs. Cell Number in Vial (cells/1ml)   4.7 x 10^6
Viability at cell freezing (%)   89.4 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   87.4 Additional information   
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