JCRB9061 PA-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB9061 | Cell Name | PA-1 |
|---|---|---|---|
| Profile | Human ovarian teratocarcinoma. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 12 |
| Identity | available | Tissue for Primary Cancer | teratocarcinoma |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ascitic fluid cells, ovarian teratocarcinoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | ascitic fluid cells, ovarian teratocarcinoma |
| Classify | tumor | Established by | Giovanella,B.C. |
| Registered by | Regulation for Distribution | ||
| Comment | May be prepared from CRL1572. | Year | 1987 |
| Medium | MEM w/ non essential amino acids + FCS 10% | Methods for Passages | Cells are treated with 0.02% EDTA and 0.25% trypsin |
| Cell Number on Passage | 1/4 - 1/10 | Race | Caucasian |
| CO2 Conc. | 5 % | Tissue Sampling | ascites |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | NT | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:4524119 | Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. Giovanella BC,Stehlin JS,Williams LJ Jr J Natl Cancer Inst. 1974 Mar;52(3):921-30 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB9061 | Cell Name | |
|---|---|---|---|
| LOT No. | 01212002 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum | Temperature | 37 C |
| Cell Density at Seeding | 1-2 x 10^5 cells/ml | Methods for Passages | 0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS |
| Doubling Time | ca 24 hrs | Cell Number in Vial (cells/1ml) | 3.0 x 10^6 |
| Viability at cell freezing (%) | 95 | Antibiotics Used | free |
| Passage Number | P339 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | Confirmed as human by NP, G6PD (type B), MD. |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS-base medium | CO2 Conc. | |
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB9061 | Cell Name | PA-1 |
|---|---|---|---|
| LOT No. | 031695 | Lot Specification | distribution |
| Medium | Eagle's minimal essential medium with non-essential amino acids & 10% fetal bovine serum/JRHB 1B1062 | Temperature | 37 C |
| Cell Density at Seeding | 2x10^4 cells/sq.cm | Methods for Passages | Rince cells once with PBS(-) and treated with 0.25% Trypsin and 0.02% EDTA |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.7x10^6 |
| Viability at cell freezing (%) | 98.7 | Antibiotics Used | free |
| Passage Number | P339 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:9,10,13 D7S820:9 D16S539:9,12 VWA:15,17 TH01:7,9 AM:X TPOX:11 CSF1PO:9,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB9061 | Cell Name | PA-1 |
|---|---|---|---|
| LOT No. | 092288 | Lot Specification | distribution |
| Medium | MEM w/ non essential amino acids + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 1/4 - 1/10 | Methods for Passages | 0.02% EDTA and 0.25% trypsin. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.5x10^6 |
| Viability at cell freezing (%) | 93% | Antibiotics Used | free |
| Passage Number | P339 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |