JCRB1209 MT-1

Cell information

Cell type：virus production cells (View Pricing Information)

JCRB No.   JCRB1209 Cell Name   MT-1
Profile   Lymphoid cell line from adult T cell leukemia. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history   ATL patient. Metastasis   
Tissue Metastasized    Genetics   Chr no. = 45 with a 14q+.
Life Span   infinite Crisis PDL   
Morphology   lymphoid Character   low level HTLV-I producer.
Classify   tumor Established by   Miyoshi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI 1640 medium with 20% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage   Split ratio = 1/2 - 1/3. Race   Japanese
CO2 Conc.   5% Tissue Sampling   peripheral blood
Tissue Type   ATL cell

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 +
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 + HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_M518
Reference
Pubmed id:7031654Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera.
Hinuma Y,Nagata K,Hanaoka M,Nakai M,Matsumoto T,Kinoshita KI,Shirakawa S,Miyoshi I
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6476-80
Pubmed id:6966589A novel T-cell line derived from adult T-cell leukemia.
Miyoshi I,Kubonishi I,Sumida M,Hiraki S,Tsubota T,Kimura I,Miyamoto K,Sato J
Gan. 1980 Feb;71(1):155-6

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1209 Cell Name   MT-1
LOT No.   07192013 Lot Specification   distribution3
Medium   RPMI1640 medium(GIBCO catalog no.11875) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Dilution,(1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.05x10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   p16* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:8
D7S820:11,13
D16S539:9
VWA:17,18
TH01:8,9
AM:X,Y
TPOX:8,9
CSF1PO:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1209 Cell Name   MT-1
LOT No.   12102007 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   Before cell dencity reaches 1x10^6/ml,dilute 2x10^5/ml
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.1x10^6
Viability at cell freezing (%)   96.1 Antibiotics Used   free
Passage Number   p16* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1209 Cell Name   MT-1
LOT No.   12172007 Lot Specification   distribution2
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   2x10^5 cells/ml Methods for Passages   Before cell dencity reaches 1x10^6/ml,dilute 2x10^5/ml
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.9x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p18* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1209 Cell Name   MT-1
LOT No.   07042013 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO catalog no.11875) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Dilution,(1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.35x10^6
Viability at cell freezing (%)   98.6 Antibiotics Used   free
Passage Number   p12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:8
D7S820:11,13
D16S539:9
VWA:17,18
TH01:8,9
AM:X,Y
TPOX:8,9
CSF1PO:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1209 Cell Name   MT-1
LOT No.   07082013 Lot Specification   distribution2
Medium   RPMI1640 medium(GIBCO catalog no.11875) with 20% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Dilution,(1/2-1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.01x10^6
Viability at cell freezing (%)   99.7 Antibiotics Used   free
Passage Number   p13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:8
D7S820:11,13
D16S539:9
VWA:17,18
TH01:8,9
AM:X,Y
TPOX:8,9
CSF1PO:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
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