JCRB1208 HM-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1208 Cell Name   HM-1
Profile   Hamster macrophage cell line from Syrian hamster transplanted with human umbilical cord leukocytes. Other Name   
Animal   hamster, Syrian Strain   Syrian
Genus   Mesocricetus Species   auratus
Sex    Age   
Identity   not done Tissue for Primary Cancer   hemo-lymphocytic
Case history   human umbilical cord leukocytes had been transplanted Metastasis   
Tissue Metastasized    Genetics   hamster cell
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   macrophage
Classify   normal Established by   Miyoshi,I.
Registered by   Miyoshi,I. Regulation for Distribution   
Comment   Cell growth is slow. When cell growth becomes faster, please check a characeteristics of the cell. Year   2007
Medium   RPMI 1640 medium with 10 % fetal calf serum Methods for Passages   dilution
Cell Number on Passage   1/2 - 1/3 Race   
CO2 Conc.   5% Tissue Sampling   lymph node
Tissue Type   enlarged axillary lymph node

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2948
Reference
Pubmed id:657122Establishment and characterization of two hamster macrophage cell lines.
Miyoshi I,Hiraki S,Kubonishi I,Matsuda Y,Nakayama T,Kishimoto H,Masuji H,Kimura I
Cancer Lett. 1978 May;4(5):253-7

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1208 Cell Name   HM-1
LOT No.   09032007 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   3.2x10^5 cells/ml Methods for Passages   subculture by dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.2x10^6
Viability at cell freezing (%)   92.3 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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