JCRB9058 RPMI 2650

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9058 Cell Name   RPMI 2650
Profile   quasi-diploid squamous carcinoma Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   nasal septum
Case history    Metastasis   
Tissue Metastasized    Genetics   quasi-diploid squamous carcinoma, nasal septum.
Life Span    Crisis PDL   
Morphology    Character   quasi-diploid squamous carcinoma, nasal septum.
Classify    Established by   Moore,G.E.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 30. Year   1986
Medium   Eagle's minim. essential medium with non-essential amino acids,Earle's BSS and 10%fetal bovine serum Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   nasal septum
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 NT
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1664
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 909700
Reference
Pubmed id:841332Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:5831238Human tumor cell line with a quasi-diploid karyotype (RPMI 2650).
Moorhead PS
Exp Cell Res. 1965 Aug;39(1):190-6
Pubmed id:14123677STUDIES OF A HUMAN TUMOR CELL LINE WITH A DIPLOID KARYOTYPE.
MOORE GE,SANDBERG AA
Cancer. 1964 Feb;17():170-5

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9058 Cell Name   
LOT No.   041498 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   1.5 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   7.4 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   P30 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9058 Cell Name   RPMI 2650
LOT No.   11172017 Lot Specification   distribution
Medium   MEM(GIBCO cat.No.11095-085) with MEM non essential amino acid solution (GIBCO cat.No.11140-050) and 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012-500ML,Lot#12J396) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.99x10^6
Viability at cell freezing (%)   82.11 Antibiotics Used   free
Passage Number   p31 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:11,12
D7S820:8,11
D16S539:11,12
VWA:16,18
TH01:6,8
AM:X,Y
TPOX:8
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   36.9 Additional information   
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