JCRB1196 KMS-20

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1196 Cell Name   KMS-20
Profile   human myeloma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   65
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   small round-cell morphology, part is multinucleate cell.
Classify   tumor Established by   Otsuki, T.
Registered by   Otsuki, T. Regulation for Distribution   
Comment    Year   2006
Medium   RPMI1640 medium with 10% featal bovine serum. Methods for Passages   Subculture twice a week by dilution(1/3-1/5).
Cell Number on Passage   50000 cells / mL Race   Japanese
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   myeloma(IgGk)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2990
Reference
Pubmed id:15491288Expression of protein gene product 9.5 (PGP9.5)/ubiquitin-C-terminal hydrolase 1 (UCHL-1) in human myeloma cells.
Otsuki T,Yata K,Takata-Tomokuni A,Hyodoh F,Miura Y,Sakaguchi H,Hatayama T,Hatada S,Tsujioka T,Sato Y,Murakami H,Sadahira Y,Sugihara T
Br J Haematol. 2004 Nov;127(3):292-8

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1196 Cell Name   
LOT No.   05302011 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   0.6 - 1.3 x 10^5 cells/mL Methods for Passages   Simple dilution
Doubling Time   approx. 45 hrs. Cell Number in Vial (cells/1ml)   4.1 x 10^6
Viability at cell freezing (%)   94.5 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   Yes
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1196 Cell Name   KMS-20
LOT No.   06222007 Lot Specification   distribution2
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   1.0x10^5 cells/ml Methods for Passages   subculture by dilution(pipetting or scraping)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.5x10^6
Viability at cell freezing (%)   94.2 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:8
D7S820:12
D16S539:9
VWA:17
TH01:7
AM:X
TPOX:8,9
CSF1PO:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1196 Cell Name   KMS-20
LOT No.   06142007 Lot Specification   distribution
Medium   RPMI1640(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   4.0x10^5 cells/ml Methods for Passages   subculture by dilution(pipetting or scraping)
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.2x10^6
Viability at cell freezing (%)   83.5 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,13
D13S317:8
D7S820:12
D16S539:9
VWA:17
TH01:7
AM:X
TPOX:8,9
CSF1PO:13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1196 Cell Name   KMS-20
LOT No.   05292015 Lot Specification   distribution
Medium   RPMI1640 medium with 10% featal bovine serum (FBS; GIBCO Cat. # 10091). Temperature   37 C
Cell Density at Seeding   3 - 6 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). Split ratio = 1/3 - 1/5.
Doubling Time   approx. 56 hrs. Cell Number in Vial (cells/1ml)   3.0 x 10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   Unknown (5 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B). MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,13
D13S317:8
D7S820:12
D16S539:9
VWA:17
TH01:7
AM:X
TPOX:8,9
CSF1PO:12,13
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   
Cell No.   JCRB1196 Cell Name   KMS-20
LOT No.   06012018 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1 - 3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   2 - 3 days. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   80 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   80 Additional information   
Cell No.   JCRB1196 Cell Name   KMS-20
LOT No.   02182022 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   1.3 - 1.5 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time    Cell Number in Vial (cells/1ml)   8.9 x 10^5
Viability at cell freezing (%)   73.3 Antibiotics Used   free
Passage Number    PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   74.2 Additional information   
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