JCRB9056 Y-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9056 Cell Name   Y-1
Profile   adrenocortical tumor, steroid-secreting, (LAF1 mouse) Other Name   
Animal   mouse Strain   
Genus   Mus Species   musculus
Sex    Age   
Identity    Tissue for Primary Cancer   adrenal cortex
Case history    Metastasis   
Tissue Metastasized    Genetics   adrenal tumor
Life Span    Crisis PDL   
Morphology    Character   adrenal tumor
Classify    Established by   Yasumura,Y. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 79. Year   
Medium   Ham's F10 medium 82.5% with 15% horse serum and 2.5% fetal calf serum. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   adrenal tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0585
Reference
Pubmed id:3169015Microtubule-assembly inhibitor protein. Its distribution, localization and physicochemical properties.
Kotani S,Ikai A,Kawai G,Maekawa S,Yokoyama S,Sakai H
Eur J Biochem. 1988 Oct 1;176(3):573-80
Pubmed id:3481365Comparison of a major heat-stable microtubule-associated protein in HeLa cells and 190-kDa microtubule-associated protein in bovine adrenal cortex.
Murofushi H,Kotani S,Aizawa H,Maekawa S,Sakai H
J Biochem. 1987 Nov;102(5):1101-12
Pubmed id:5930699Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype.
Yasumura Y,Buonassisi V,Sato G
Cancer Res. 1966 Mar;26(3):529-35
Pubmed id:13441719In vitro response of functional experimental adrenal tumors to corticotropin ACTH.
COHEN AI,BLOCH E,CELOZZI E
Proc Soc Exp Biol Med. 1957 Jun;95(2):304-9
Pubmed id:13444454Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats.
COHEN AI,FURTH J,BUFFETT RF
Am J Pathol. 1957 Jul-Aug;33(4):631-51

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9056 Cell Name   Y-1
LOT No.   110796 Lot Specification   distribution
Medium   Ham's F10 medium with 15% horse serum and 2.5% fetal bovine serum (JRH 5G2126/JRH 1B1062). Temperature   37 C
Cell Density at Seeding   1-2x10^5 cells/ml Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.3x10^6
Viability at cell freezing (%)   99.0 Antibiotics Used   free
Passage Number   P53 PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, LDH, NP (mouse)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9056 Cell Name   Y-1
LOT No.   081694 Lot Specification   distribution
Medium   Ham's F-10 medium with 2.5% fetal bovine serum and 15% horse serum. Temperature   37 C
Cell Density at Seeding   2-8x10^5 cells/10cm dish Methods for Passages   Cells were treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   P53 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   mouse (NP,G6PD,PepB,MPI,AST,LD)
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9056 Cell Name   Y-1
LOT No.   122589 Lot Specification   distribution
Medium   Ham's F10 : 82.5%, horse serum : 15%, FCS : 2.5%. Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.1x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P3 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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