JCRB9048 MOLT-3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9048 Cell Name   MOLT-3
Profile   leukemia, acute lymphoblastic Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   19
Identity   available Tissue for Primary Cancer   hemo-lymphocytic
Case history    Metastasis   
Tissue Metastasized    Genetics   Peripheral blood, acute lymphoblastic leukemia
Life Span    Crisis PDL   
Morphology    Character   Peripheral blood, acute lymphoblastic leukemia
Classify    Established by   Minowada,J.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CRL1552. Year   1986
Medium   RPMI1640 medium with 10% fetal calf serum Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtainable.
Cell Number on Passage   5-6 x 10^5 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0624
Reference
Pubmed id:2846092Immunophenotypic and cytogenetic analysis of Molt-3 and Molt-4: human T-lymphoid cell lines with rearrangement of chromosome 7.
Greenberg JM,Gonzalez-Sarmiento R,Arthur DC,Wilkowski CW,Streifel BJ,Kersey JH
Blood. 1988 Nov;72(5):1755-60
Pubmed id:6991122The differentiation and function of human T lymphocytes.
Reinherz EL,Schlossman SF
Cell. 1980 Apr;19(4):821-7
Pubmed id:4366935The presence of the Epstein-Barr viral genome in human lymphoblastoid B-cell lines and its absence in a myeloma cell line.
Minowada J,Nonoyama M,Moore GE,Rauch AM,Pagano JS
Cancer Res. 1974 Aug;34(8):1898-903
Pubmed id:4567231Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.
Minowada J,Onuma T,Moore GE
J Natl Cancer Inst. 1972 Sep;49(3):891-5

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9048 Cell Name   
LOT No.   12272001 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal bovine serum Temperature   37 C
Cell Density at Seeding   5-13 x 10^4 cells/ml Methods for Passages   dilution
Doubling Time    Cell Number in Vial (cells/1ml)   6 x 10^6
Viability at cell freezing (%)   81.3 Antibiotics Used   free
Passage Number   P9* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS-RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9048 Cell Name   MOLT-3
LOT No.   100995 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat inactivated fetal bovine serum (ICN Flow 1010183) Temperature   37 C
Cell Density at Seeding   2.5x10^5 cells/ml Methods for Passages   dilution, @1-3 x 10^5 cells/ml
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.45x10^6
Viability at cell freezing (%)   85.9 Antibiotics Used   free
Passage Number   P9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(Type B), NP and LDH examined. Human.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB9048 Cell Name   MOLT-3
LOT No.   081693 Lot Specification   distribution
Medium   RPMI1640 medium wth 10% fetal bovine serum (ICN Flow 1059023, or Hyclone 115103L) Temperature   37 C
Cell Density at Seeding   2-7x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.5x10^6
Viability at cell freezing (%)   66.6 Antibiotics Used   free
Passage Number   *P10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9048 Cell Name   MOLT-3
LOT No.   032291 Lot Specification   distribution
Medium   RPMI1640 + FCS 10% Temperature   37 C
Cell Density at Seeding   5-6 x 10^5/ml Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   *P9 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9048 Cell Name   MOLT-3
LOT No.   021787 Lot Specification   distribution
Medium   RPMI1640 medium with 10% fetal calf serum Temperature   37 C
Cell Density at Seeding   5-6 x 10^5 cells/ml Methods for Passages   Dilution. A maximum of 1-2 x 10^6 cells/ml is obtainable.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD,LD,NP
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9048 Cell Name   MOLT-3
LOT No.   10252021 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500) Temperature   37 C
Cell Density at Seeding   0.9 - 3.3 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture)
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   3.1 x 10^6
Viability at cell freezing (%)   91.3 Antibiotics Used   free
Passage Number   Unknown (9 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:12
D13S317:12,13
D7S820:8,10
D16S539:11,14
VWA:17
TH01:6,8
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83.6 Additional information   
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