JCRB9045 Detroit 539

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9045 Cell Name   Detroit 539
Profile   Down's syndrome, skin Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history   human skin cell line established from down's syndrome patient. Metastasis   
Tissue Metastasized    Genetics   Skin, Down's syndrome, female
Life Span   finite Crisis PDL   
Morphology    Character   Skin, Down's syndrome, female
Classify    Established by   Simpson,W.E. et al.
Registered by    Regulation for Distribution   Following rules from ATCC
Comment   Prepared from CCL 84. Population doubling is unknown. These cells have a total life expectancy of about 25-30 serial subcultures from tissue of origin. Year   1986
Medium   MEM,NEAA,Na pyruvate,lactalbumin hydrolysate(0.1%)+FCS 10% Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin
Cell Number on Passage   split ratio=1/2 - 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2431
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9045 Cell Name   Detroit 539
LOT No.   062289 Lot Specification   distribution
Medium   MEM,NEAA,Na pyruvate,lactalbumin hydrolysate(0.1%)+FCS 10% Temperature   37
Cell Density at Seeding   1/2 - 1/4 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.6x10^5
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P17 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   NT
Sterility: FUNGI   NT Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   Vials are labelled as "Det 539"
Cell No.   JCRB9045 Cell Name   Detroit 539
LOT No.   02012016 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids (0.1 mM each), 1 mM sodium pyruvate, 0.1% lactalbumin hydrolysate and 10% fetal bovine serum (FBS; GIBCO Cat. # 10091) Temperature   37 C
Cell Density at Seeding   2.0 - 2.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Split ratio 1:6.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   1.5 x 10^6
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   P19 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   92.6 Additional information   
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