JCRB9041 T98G

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB9041 Cell Name   T98G
Profile   Human glioblastoma multiforma tumor. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   61
Identity   available Tissue for Primary Cancer   neural
Case history   glioblastoma multiform tumor Metastasis   
Tissue Metastasized    Genetics   G1 arrested when crowded
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   anchorage-independent,good to study cell prolife.
Classify   transformed Established by   Hayflick,L.
Registered by    Regulation for Distribution   
Comment   Prepared from CRL1690. The cell line was identified to be unique. Protease resistant prion protein detected. Year   1986
Medium   Eagle's minimal essential medium with non-essential amino acids , Na pyruvate, and 10% FCS. Methods for Passages   Cells are treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/10 - 1/3 Race   caucasian
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0556
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link. 687586
Reference
Pubmed id:15483263Propagation of a protease-resistant form of prion protein in long-term cultured human glioblastoma cell line T98G.
Kikuchi Y,Kakeya T,Sakai A,Takatori K,Nakamura N,Matsuda H,Yamazaki T,Tanamoto KI,Sawada JI
J Gen Virol. 2004 Nov;85(Pt 11):3449-3457
Pubmed id:12081137G1-dependent prion protein expression in human glioblastoma cell line T98G.
Kikuchi Y,Kakeya T,Yamazaki T,Takekida K,Nakamura N,Matsuda H,Takatori K,Tanimura A,Tanamoto K,Sawada J
Biol Pharm Bull. 2002 Jun;25(6):728-33
Pubmed id:222778T98G: an anchorage-independent human tumor cell line that exhibits stationary phase G1 arrest in vitro.
Stein GH
J Cell Physiol. 1979 Apr;99(1):43-54

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB9041 Cell Name   
LOT No.   10112011 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 0.1 mM non-essential amino acids, 1 mM sodium pyruvate and 10% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. Passage twice a week.
Doubling Time   approx. 23 hrs. Cell Number in Vial (cells/1ml)   7.6 x 10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   P433 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM with supplements. CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9041 Cell Name   T98G
LOT No.   022095 Lot Specification   FDSC/distribution
Medium   Eagle's minimal essential medium with non-essential aa(1mM Na-pyrvate) and 10% fetal bovine serum. Temperature   37 C
Cell Density at Seeding   4.3-7.1x10^5 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.8x10^5
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   P273 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9041 Cell Name   T98G
LOT No.   121592 Lot Specification   distribution
Medium   Eagle`s MEM with non-essential amino acids,1mM sodium pyruvate and fetal bovine serum(Hyclone1115975 Temperature   37 C
Cell Density at Seeding   9.5x10^4 cells/ml Methods for Passages   Cells are treated with 0.25% trypsin and 0.01% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   95.5 Antibiotics Used   free
Passage Number   P433 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9041 Cell Name   T98G
LOT No.   092691 Lot Specification   distribution
Medium   MEM + NEAA + Na pyruvate + FCS10% Temperature   37 C
Cell Density at Seeding   1/10 - 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P430 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9041 Cell Name   T98G
LOT No.   122188 Lot Specification   distribution
Medium   MEM,non essential amino acids,Na pyruvate + FCS10% Temperature   37
Cell Density at Seeding   1/10 - 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.8x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P429 PDL   
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP,LD,G6PD
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9041 Cell Name   T98G
LOT No.   012387 Lot Specification   distribution
Medium   MEM,non essential amino acids,Na pyruvate + FCS10% Temperature   37
Cell Density at Seeding   1/10 - 1/3 Methods for Passages   0.02% EDTA and 0.25% trypsin.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   P428 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   AST,G6PD,LD,MD,MPI,NP,PEPB
Chromosome Mode    Chromosome Information   NT
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9041 Cell Name   
LOT No.   071796 Lot Specification   distribution
Medium   Eagle's MEM with non-essential amino acids, 1 mM Na-pyruvate and 10% FCS (lot; BioWhittaker 3M0581) Temperature   37 C
Cell Density at Seeding   2-7 x 10^4 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   6.6 x 10^5
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   P434 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   confirmed as human by NP, G6PD (type B), MD
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB9041 Cell Name   T98G
LOT No.   12132024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with non-essential amino acids, 1 mM sodium pyruvate and 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   2.4 - 2.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 24 hrs. Cell Number in Vial (cells/1ml)   1.0 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P433 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:13
D7S820:9,10
D16S539:13
VWA:17,20
TH01:7,9.3
AM:X,Y
TPOX:8
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)   94 Additional information   
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